QUANTIFICATION OF PSEUDOPROTODIOSCIN FROM ASPARAGUS COCHINCHINENSIS (LOUR.) MERR. BY HPLC-UV

Nguyễn Thị Phương; Hoàng Thị Tuyết; Vi Thị Tuyến; Phùng Thị Hoài Thu; Nguyễn Hữu Thiện; Lê Thị Phượng; Nguyễn Thị Hạnh

doi:10.34238/tnu-jst.8326

QUANTIFICATION OF PSEUDOPROTODIOSCIN FROM ASPARAGUS COCHINCHINENSIS (LOUR.) MERR. BY HPLC-UV

About this article

Received: 13/07/23 Revised: 30/08/23 Published: 13/09/23

Authors

1. Nguyen Thi Phuong , Center for application of science and technology in Medicinal Material
2. Hoang Thi Tuyet, Center for application of science and technology in Medicinal Material
3. Vi Thi Tuyen, Center for application of science and technology in Medicinal Material
4. Phung Thi Hoai Thu, Center for application of science and technology in Medicinal Material
5. Nguyen Huu Thien, Dong A Science and Technologt Joint Stock Company
6. Le Thi Phuong, Dong A Science and Technologt Joint Stock Company
7. Nguyen Thi Hanh, Dong A Science and Technologt Joint Stock Company

Abstract

The objective of this study was to develop and validate for quantification of pseudoprotodioscin from Asparagus cochinchinensis (Lour.) Merr. by HPLC coupled with UV-Vis detector. The method was carried out by using a Lightversil C18 (25 cm x 4.6 mm, 5 µm) column with various gradients of solvent system of water – acetonitrile, the flow rate of 1 mL/min. For detection of pseudoprotodioscin, the absorption wavelength of detection was set at 203 nm. Results showed that the calibration curve displayed a good linear relationship (R²=1). The validation results displayed the good accuracy, repeatability, linearity, and selectivity according to the ICH guideline. The limits of detection (LOD) and quantification (LOQ) were 2.13 and 6.45 µg/ml for pseudoprotodioscin, respectively. The content of pseudoprotodioscin in A.cochinchinensis (Lour.) Merr.) collected in Vietnam ranged from 0.020% to 0.206%. The results provide useful information for the quality assessment of A.cochinchinensis (Lour.) Merr. in Vietnam. The method is qualified for the determination of the content of pseudoprotodioscin in A.cochinchinensis (Lour.) Merr.

Keywords

Quantification; Asparagus cochinchinensis (Lour.) Merr.; Vietnam; Pseudoprotodioscin; HPLC-UV

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References

[1] D. H. Bich, Medicinal plants and animals in Vietnam. Science and technics publishing house, 2009, pp. 863-866.

[2] M. Wang, S. Wang, W. Hu, Z. Wang, B. Yang, and H. Kuang, “Asparagus cochinchinensis: A review of its botany, traditional uses, phytochemistry, pharmacology, and applications,” Frontiers in Pharmacology, vol. 13, pp. 1068858-1068858, 2022.

[3] B. Sun, D. Yang, Y. Z. Yin, and J. Xiao, “Estrogenic and anti-inflammatory effects of pseudoprotodioscin in atherosclerosis-prone mice: Insights into endothelial cells and perivascular adipose tissues,” European Journal of Pharmacology, vol. 869, 2020, Art. no. 172887.

[4] Y. Gai, Y. Li, Z. Xu, and J. Chen, “Pseudoprotodioscin inhibits SREBPs and microRNA 33a/b levels and reduces the gene expression regarding the synthesis of cholesterol and triglycerides,” Fitoterapia, vol. 139, 2019, Art. no. 104393.

[5] M. A. Siddiqui, Z. Ali, A. G. Chittiboyina, I. A. Khan, “Hepatoprotective effect of steroidal glycosides from Dioscorea villosa on hydrogen peroxide-induced hepatotoxicity in HepG2 cells,” Frontiers in pharmacology, vol. 9, p. 797, 2018.

[6] M. Dong, X. Z. Feng, L. J. Wu, B. X. Wang, T. Ikejima, “Two new steroidal saponins from the rhizomes of Dioscorea panthaica and their cytotoxic activity,” Planta medica, vol. 67, no. 09, pp. 853-857, 2001.

[7] HongKong Chinese Materia Medica Standards (HKCMMS) Office, Asparagi Radix, vol. 9, pp. 43-55, 2009.

[8] Ministry of Health, Vietnamese Pharmacopoeia V. Medical Publishing House, Hanoi, Vietnam, 2017, p. 1339.

[9] ICH, Validation of analytical procedures: Text and methodology Q2(R1), ICH Hamonised Tripartite Guideline, pp. 1-13, 2005.

[10] L. Guo, S. L. Zeng, Y. Zhang, P. Li, and E. H. Liu, “Comparative analysis of steroidal saponins in four Dioscoreae herbs by high performance liquid chromatography coupled with mass spectrometry,” Journal of Pharmaceutical and Biomedical Analysis, vol. 117, pp. 91-98, 2016.

DOI: https://doi.org/10.34238/tnu-jst.8326

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