EVALUATION OF SOME CHEMICAL COMPONENTS AND BIOLOGICAL ACTIVITIES OF Cordyceps militaris HYBRID STRAINS

Nguyễn Trường Khoa; Nguyễn Đình Việt; Trần Duy Cường; Lê Thị Khánh; Trần Thị Phương Thuý; Hà Huy Công; Nguyễn Phương Đại Nguyên; Dương Văn Cường; Lê Thị Tươi

doi:10.34238/tnu-jst.10271

EVALUATION OF SOME CHEMICAL COMPONENTS AND BIOLOGICAL ACTIVITIES OF Cordyceps militaris HYBRID STRAINS

About this article

Received: 03/05/24 Revised: 10/06/24 Published: 24/06/24

Authors

1. Nguyen Truong Khoa, Agricultural Genetics Institute
2. Nguyen Dinh Viet, Hanoi National University of Education
3. Tran Duy Cuong, Agricultural Genetics Institute
4. Le Thi Khanh, Hanoi National University of Education
5. Tran Thi Phuong Thuy, Hanoi National University of Education
6. Ha Huy Cong, Hanoi National University of Education
7. Nguyen Phuong Dai Nguyen, Tay Nguyen University
8. Duong Van Cuong, TNU - University of Agriculture and Forestry
9. Le Thi Tuoi , Hanoi National University of Education

Abstract

Cordyceps militaris is an insect parasitic fungus of high pharmacological and economic value. The process of artificially cultivating this fungus has been successful and is increasingly expanding, requiring strain stability. Previous research results showed that single-spore hybrid fungal strains ensure stability. In this study, ascospores from different parental sources were genotyped by MAT1-1-1 and MAT1-2-1 genes and hybridized on rice-pupa media. The morphology and fruiting body yield of 8 hybrid strains showed that hybrid CT1 and CT2 had long fruiting body morphology and dry yield of 4.4 and 5.0 g/jar, respectively. Meanwhile, the CT4 hybrid fruit body extract has cordycepin, adenosine, and total phenolic content, as well as DPPH free radical scavenging activity nearly twice as high as the CT1 and CT2 hybrids. The tyrosinase enzyme inhibitory activity of CT2 hybrid fruit body extract (59.0%) was higher than that of other hybrids. The results show that hybrid strains CT1, CT2, and CT4 are potential strains for breeding strategies to develop new strains with high yield and quality.

Keywords

Biological activity; Chemical component; Cordycepin; Cordyceps militaris; Hybrid strain

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DOI: https://doi.org/10.34238/tnu-jst.10271

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