THE INHIBITORY EFFECTS ON MIGRATION AND INVASION OF LIVER CANCER CELLS BY ETHANOL EXTRACT FROM AMPELOPSIS CANTONIENSIS ON 2D AND 3D CULTURE MODELS

Cấn Đình Quang Hưng; Lê Thị Ngọc Thuý; Hoàng Việt; Ngô Hồng Phương; Đỗ Thị Thu Huyền; Trần Trung Anh; Hoàng Văn Hùng; Nguyễn Phú Hùng; Lê Thị Thanh Hương

doi:10.34238/tnu-jst.10423

THE INHIBITORY EFFECTS ON MIGRATION AND INVASION OF LIVER CANCER CELLS BY ETHANOL EXTRACT FROM AMPELOPSIS CANTONIENSIS ON 2D AND 3D CULTURE MODELS

About this article

Received: 20/05/24 Revised: 17/06/24 Published: 17/06/24

Authors

1. Can Dinh Quang Hung, TNU - University of Sciences
2. Le Thi Ngoc Thuy, TNU - University of Sciences
3. Hoang Viet, TNU - University of Sciences
4. Ngo Hong Phuong, TNU - University of Sciences
5. Do Thi Thu Huyen, TNU - University of Sciences
6. Tran Trung Anh, Thai Nguyen National Hospital
7. Hoang Van Hung, TNU - Center for Interdisciplinary Science and Education
8. Nguyen Phu Hung, TNU - Center for Interdisciplinary Science and Education
9. Le Thi Thanh Huong , TNU - University of Sciences

Abstract

The Ampelopsis cantoniensis (A. Cantoniensis) is distributed in various regions of Vietnam and is commonly used in traditional medicine to prevent and treat various diseases. In this study, ethanol extract from A. cantoniensis leaves was evaluated for its impact on migration, invasion of HepG2 liver cancer cells using methods analyzing changes in border zone diameter, and analysis of tumorsphere invasion ability. The results showed that the A. cantoniensis ethanol extract inhibited the migration and invasion ability of HepG2 liver cancer cells in a dose-dependent manner. In the control group, the width of the border after 24 hours was narrowed to 68.1 ± 7.2%, and after 72 hours to 19.7 ± 2.4%. At a concentration of 50 µg/mL, the migration significantly decreased, and the width of the border narrowed more slowly compared to the control, 81.4 ± 7.5% after 24 hours and 71.7 ± 6.8% after 72 hours (p < 0.001). When cells were treated with a concentration of 100 µg/mL, the change in width of the border compared to the pre-treatment time (0 h) was minimal. The extraction of A. Cantoniensis decreased the adhesion and invasion abilities of tumorspheres and induced a pattern of cellular apoptosis. Evaluation on both 2D and combined 3D culture models showed that A. cantoniensis had a strong inhibitory effect on adhesion and invasion of HepG2 tumorspheres.

Keywords

Liver cancer; Cell migration; Cell invasion; Ampelopsis cantoniensis; Ethanol extract

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DOI: https://doi.org/10.34238/tnu-jst.10423

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