PHYTOCHEMICAL INVESTIGATION AND ATIOXIDANT ACTIVITY OF ETHANOLIC EXTRACT OF Blumea densiflora var. hookeri  (C.B. Clarke ex Hook.f.) C.C. Chang & Y.Q. Tseng LEAVES

Hoàng Thị Bình; Đỗ Trần Thẩm Thuý; Huỳnh Thị Hương Trầm; Phạm Trần Thị Vân Khánh; Đặng Văn Văn; Nguyễn Thị Trà; Lê Ngọc Quỳnh Giao; Đàm Anh Sơn; Nguyễn Văn Ngọc

doi:10.34238/tnu-jst.10522

PHYTOCHEMICAL INVESTIGATION AND ATIOXIDANT ACTIVITY OF ETHANOLIC EXTRACT OF Blumea densiflora var. hookeri (C.B. Clarke ex Hook.f.) C.C. Chang & Y.Q. Tseng LEAVES

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Received: 03/06/24 Revised: 26/09/24 Published: 27/09/24

Authors

1. Hoang Thi Binh, Dalat University
2. Do Tran Tham Thuy, Dalat University; Tay Nguyen research center of medicinal materials - National Institute of medicinal materials
3. Huynh Thi Huong Tram, Dalat University
4. Pham Tran Thi Van Khanh, Dalat University
5. Dang Van Van, Dalat University
6. Nguyen Thi Tra, Dalat University
7. Le Ngoc Quynh Giao, Dalat University
8. Dam Anh Son, Dalat University
9. Nguyen Van Ngoc , Dalat University

Abstract

The study aimed to evaluate the efficiency of high extraction from the leaves of Blumea densiflora var. hookeri, distributed in Lam Dong by using ethanol solvent. It also investigated the chemical composition and antioxidant activity of the ethanol extract obtained from this species. The results show that the efficiency of ethanol extraction by the soxhlet extraction method of B. densiflora var. hookeri was 9.62%. The chemical composition of the ethanol extract was characterized according to the method of Nguyen Van Dan and Nguyen Viet Tuu (1985), combined with the method of Nguyen Kim Phi Phung (2007), revealing 7 groups of compounds, including alkaloids, terpenoid-steroids, flavonoids, glycosides, cardiac glycosides, tannins, and coumarin. The total polyphenol content recorded from the leaf extract was 5.87 (mgGAE/g dry weight). Through investigation of antioxidant activity using the DPPH free radical neutralization method, it was shown that the ethanol extract of B. densiflora var. hookeri exhibits antioxidant activity with an IC₅₀value of 4532.28 (μg/ml). The findings of this research not only add to our understanding of the biochemical aspects of this plant species but also foster its exploitation, application, conservation, and development in human life.

Keywords

Antioxidant activity; Blumea densiflora var. hookeri; Ethanol extract; Phytochemistry; Lam Dong

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DOI: https://doi.org/10.34238/tnu-jst.10522

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