PHYLOGENETIC RELATIOSHIP OF PANAX SPECIES  IN VIENAM USING MIG-SEQ METHOD

Hoàng Thị Bình; Lê Minh Tâm; Phạm Quang Tuyến; Trịnh Ngọc Bon; Vũ Quốc Luận; Nguyễn Văn Ngọc

doi:10.34238/tnu-jst.10537

PHYLOGENETIC RELATIOSHIP OF PANAX SPECIES IN VIENAM USING MIG-SEQ METHOD

About this article

Received: 04/06/24 Revised: 17/12/24 Published: 18/12/24

Authors

1. Hoang Thi Binh, Dalat University
2. Le Minh Tam, Vaccine Company Limited of Dalat Pasteur
3. Pham Quang Tuyen, Silviculture Research Institute - Forest Science Institute of Vietnam
4. Trinh Ngoc Bon, Silviculture Research Institute - Forest Science Institute of Vietnam
5. Vu Quoc Luan, Tay Nguyen Institute for Scientific Research - Vietnam Academy of Scientific and Technology
6. Nguyen Van Ngoc , Dalat University

Abstract

This study aim to examine the phylogenetic relationship between Panax species (Araliaceae) of Vietnam using MIG-seq method based on Next Generation Sequencing platform. A total of 29 Vietnamese Panax samples, including five species, two varieties, and one sample of Panax ginseng as an outgroup, were utilized. The MIG-seq method generated a dataset of 6821 SNPs, which was used to reconstruct a phylogenetic tree using maximum likelihood. The tree demonstrated the phylogenetic relationship between Panax species with high resolution and strongly supported monophyly through bootstrap values. The results revealed that the Panax species of Vietnam comprise two main sister groups. The first group includes P. stipuleanatus and P. bipinnatifidus, while the second group comprises three species (P. pseudoginseng, Panax sp., P. vietnamensis) and two varieties (P. vietnamensis var. fuscidicus and P. vietnamensis var. langbianensis). These research findings provide a foundation for the application of the MIG-seq method in studying phylogenetic relationships of plant in Vietnam.

Keywords

Araliaceae; Classification; ISSR; Phylogeny; Sequencing

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DOI: https://doi.org/10.34238/tnu-jst.10537

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