CLONING AND DETERMINATION GENE SEQUENCES OF OUTER MEMBRANE PROTEINS OmpK AND OmpU in Vibrio parahaemolyticus N9

Ngô Thị Huyền; Đồng Văn Quyền; Trần Ngọc Kiên; Phạm Thị Tâm; Ngô Đức Mạnh; Lê Thị Tươi; Vũ Thị Bích Huyền

doi:10.34238/tnu-jst.10621

CLONING AND DETERMINATION GENE SEQUENCES OF OUTER MEMBRANE PROTEINS OmpK AND OmpU in Vibrio parahaemolyticus N9

About this article

Received: 20/06/24 Revised: 16/10/24 Published: 17/10/24

Authors

1. Ngo Thi Huyen, Vietnam Academy of Science and Technology
2. Dong Van Quyen, Vietnam Academy of Science and Technology
3. Tran Ngoc Kien, Vinschool The Harmony
4. Pham Thi Tam, Hanoi Open University
5. Ngo Duc Manh, University of Science - Vietnam National University
6. Le Thi Tuoi, Hanoi National University of Education
7. Vu Thi Bich Huyen , Hanoi National University of Education

Abstract

Vibrio parahaemolyticus is one of the dangerous pathogens of aquatic animals, including fish. The outer membrane proteins play an essential role in bacterial virulence and are potential candidates for vaccine development. In this study, the virulence of four V. parahaemolyticus strains was studied, and the result was that strain. The V. parahaemolyticus N9 has the strongest virulence with an LD₅₀of 10^6.15CFU/mL. OmpK and OmpU genes from V. parahaemolyticus N9 strain, which are coding for two outer membrane proteins. Two genes were determined the sequence of the open reading frame (ORF) with sizes of 819 bp (OmpK gene) and 1014 bp (OmpU gene), respectively. In addition, we also successfully cloned these two antigen genes OmpK and OmpU into pGEM-T and pCE2 TA vectors, respectively. This is the first step in studying vaccine development against diseases caused by V. parahaemolyticus in some marine fish species.

Keywords

OmpK; OmpU; Vibrio parahaemolyticus; Cloning; Antigen

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DOI: https://doi.org/10.34238/tnu-jst.10621

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