EVALUATION OF ANTIBACTERIAL ACTIVITY AND ABILITY TO CYTOTOXIC CANCER CELLS OF BACTERIA ISOLATED FROM  TAM GIANG LAGOON SLUDGE, THUA THIEN HUE PROVINCE

Nguyễn Thị Ngọc Anh; Trần Thị Hồng; Tạ Thị Thu Thuỷ; Vũ Kim Thoa

doi:10.34238/tnu-jst.10799

EVALUATION OF ANTIBACTERIAL ACTIVITY AND ABILITY TO CYTOTOXIC CANCER CELLS OF BACTERIA ISOLATED FROM TAM GIANG LAGOON SLUDGE, THUA THIEN HUE PROVINCE

About this article

Received: 22/07/24 Revised: 17/11/24 Published: 18/11/24

Authors

1. Nguyen Thi Ngoc Anh , Hanoi Open University; Graduate University of Science and Technology - VAST
2. Tran Thi Hong, Graduate University of Science and Technology - VAST; Mientrung Institute for Scientific Research, Vietnam National Museum of Nature - VAST
3. Ta Thi Thu Thuy, Hanoi Open University
4. Vu Kim Thoa, Hanoi Open University

Abstract

Tam Giang, a lagoon located in the Central region of Vietnam, is one of the largest in Southeast Asia, and is considered very diverse and rich in biological resources. However, there have been no studies evaluating the microflora here. This study aimed to isolate bacterial strains capable of being cultured in the Tam Giang lagoon and their potential to biosynthesize antibacterial and anti-cancer compounds. This study screened twenty-two bacterial strains with antagonistic activity against 1 - 6 test strains. An antagonistic ring diameter ranging from 5.0 - 36.3 mm, especially strain TGGD GII15 was determined to be antagonistic to the six tested strains included Bacillus cereus ATCC 11778, Staphylococcus aureus ATCC 6538, Pseudomonas putida 3326K1, Pseudomonas auroginosa ATCC 9027, Cadida albicans ATCC 10231, and Salmonella typhimurium ATCC 14028. This strain was also found to be cytotoxic to the MCF-7 breast cancer and Hep G2 liver cancer cells but not to the regular HEK 293T cells. Gene 16S rRNA sequencing results showed that strain TGGD GII15 was 99.93% similar to Bacillus amyloloquefaciens (NR code 041455.1 in NCBI). This is the first study on Vietnamese coastal lagoon microorganisms, opening up excellent prospects for exploiting valuable biological compounds from this ecosystem.

Keywords

Tam Giang Lagoon; Bacterial; Antibacterial; Anticancer; Bacillus amyloliquefaciens

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DOI: https://doi.org/10.34238/tnu-jst.10799

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