PHEOPHORBIDE A DEMONSTRATES THE ABILITY TO INHIBIT PORCINE EPIDEMIC DIARHEA VIRUS WHEN COMBINED WITH 660-NM LED LIGHT | Hiếu | TNU Journal of Science and Technology

PHEOPHORBIDE A DEMONSTRATES THE ABILITY TO INHIBIT PORCINE EPIDEMIC DIARHEA VIRUS WHEN COMBINED WITH 660-NM LED LIGHT

About this article

Received: 17/12/24                Revised: 04/03/25                Published: 05/03/25

Authors

1. Nguyen Duc Hieu Email to author, Institute of Biotechnology – Vietnam Academy of Science and Technology
2. Nguyen Dieu Linh, Institute of Biotechnology – Vietnam Academy of Science and Technology
3. Tong Quang Cong, Institute of Materials Science - Vietnam Academy of Science and Technology
4. Pham Bich Ngoc, Institute of Biotechnology – Vietnam Academy of Science and Technology; Graduate University of Science and Technology
5. Hoang Thi Thu Hang, Institute of Biotechnology – Vietnam Academy of Science and Technology; Graduate University of Science and Technology
6. Tran Quoc Tien, Institute of Materials Science - Vietnam Academy of Science and Technology

Abstract


Photodynamic therapy has been used in many viral treatments due to its ability to disrupt viral particles or inhibit viral entry and replication. Therefore, photodynamic therapy is now considered a potential method to address the rapid mutations of viruses that vaccines often fail to counter. Pheophorbide a, a naturally derived photoactive compound, is widely recognized for its effectiveness against cancer cell lines, hepatitis virus, SAR-CoV-2 and influenza viruses. In our recent study, pheophorbide a was demonstrated to inhibit porcine epidemic diarrhea virus, which is one of the most serious disease in pigs. The study showed that in vitro porcine epidemic diarrhea virus replication decreased in reciprocal proportion with the concentration of pheophorbide a. Inhibitory effects were observed to intensify from 24 to 48 hours with pheophorbide a concentrations equal to or higher than one µg/ml. Notably, at the pheophorbide a concentration of 3 µg/ml combined with LED light (660 nm, 4.17 J/cm2), pheophorbide a exhibited significant antiviral activity 48 hours after porcine epidemic diarrhea virus infection.  These findings suggest promising avenues of photodynamic therapy using pheophorbide a and 660-nm LED light in controlling porcine epidemic diarrhea virus replication.

Keywords


Photo dynamic therapy; Pheophorbide a; Alphacoronavirus; Porcine epidemic diarrhea virus; 660 nm

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DOI: https://doi.org/10.34238/tnu-jst.11727

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