ISOLATION AND STUDY OF OPTIMAL CULTURE CONDITIONS FOR NITROGEN-FIXING MICROBIOLOGICAL STRAINS

Nguyễn Thanh Hải; Nguyễn Viết Hưng; Nguyễn Thế Hùng; Nguyễn Mạnh Tuấn; Nguyễn Thị Trang; Đỗ Thị Lan; Nguyễn Văn Thuần

doi:10.34238/tnu-jst.11763

ISOLATION AND STUDY OF OPTIMAL CULTURE CONDITIONS FOR NITROGEN-FIXING MICROBIOLOGICAL STRAINS

About this article

Received: 24/12/24 Revised: 04/03/25 Published: 05/03/25

Authors

1. Nguyen Thanh Hai , TNU - University of Agriculture and Forestry
2. Nguyen Viet Hung, Vietnam Academy for Ethnic Minorities
3. Nguyen The Hung, TNU - University of Agriculture and Forestry
4. Nguyen Manh Tuan, TNU - University of Agriculture and Forestry
5. Nguyen Thi Trang, TNU - University of Agriculture and Forestry
6. Do Thi Lan, TNU - University of Agriculture and Forestry
7. Nguyen Van Thuan, TNU - University of Agriculture and Forestry

Abstract

The study focused on isolating and evaluating the nitrogen fixation ability of microbial strains from the soil of cassava and arrowroot in the northern mountainous provinces of Vietnam, and determining the culture conditions and application on organic materials. Six (6) bacterial strains with nitrogen fixation ability were isolated and tested for NH₄⁺ production on Ashby and AT media. Among them, strain N14.1 was determined to have the highest NH₄⁺ production capacity, reaching 11.2 mg/l. Based on the 16S rRNA gene sequence analysis, strain N14.1 was identified as Rhizobium petrolearium. Strain N14.1 grew well in Ashby medium with pH 7.0 ± 0.2, 72 hours, 30°C. The cell density of Rhizobium petrolearium N14.1 in composted pig manure, compost (pig manure and cassava pulp) and compost (pig manure and cassava pulp) at 3 months decreased from 11.82-14.21% compared to the initial level, but was lower than that in composted cassava pulp (29.91%) and composted cassava pulp (34.69%). This result provides an important scientific basis and application for developing nitrogen-fixing microbial preparations in sustainable agriculture.

Keywords

Arrowroot residue; Cassava pulp; Rhizobium petrolearium; Isolation; Nitrogen fixing microorganisms

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References

[1] Y. Ng. Zheng, A. A. Aaronn, Y. C. Wai, Ng. Eng-Poh, A. Rosazlin, and C. L. Tau, "Towards circular economy: Potential of microalgae–bacterial-based biofertilizer on plants," Journal of Environmental Management, vol. 349, 2024, Art. no. 119445, doi: 10.1016/j.jenvman.2023.119445.

[2] F. Y. Shomi, M. B. Uddin, and T. Zerin, "Isolation and characterization of nitrogen-fixing bacteria from soil sample in Dhaka, Bangladesh," Bangladesh Journals Online, vol. 11, no. 1, pp. 11-13, 2021, doi: 10.3329/sjm.v11i1.57145.

[3] T. H. L. Nguyen, T. M. T. Nguyen, V. H. Nguyen, Q. H. Nguyen, K. B. T. Nguyen, and T. H. T. Phan, "Biological Characteristics of Rhizobium Isolated from Soybean Nodules in Hanoi, Vietnam," VNU Journal of Science: Earth and Environmental Sciences, vol. 33, no. 1S, pp. 69-75, 2017.

[4] T. P. Tran and N. D. Cao, "Effect of nitrogen - fixing Burkholderia tropicalis & B. tropica strains, inorganic nitrogen fertilizer and foliar - fertilizer to pineapple yield and quality fruit cultivated on acid sulfate soil of Tan Phuoc district, Tien Giang province," Vietnam Journal of Agriculture and Rural Development, no. 12, pp. 23-28, 2011.

[5] H. H. Nguyen, K. T. Nguyen, N. T. Le, V. B. Nguyen, T. B. L. Nguyen, T. T. Phan, T. H. Le, and V. T. Tran, "Isolation, selection of phosphate and nitrogen fixing solubilising rhizosphere microorganisms from coffee cultivated in Dak Lak province," Vietnam Journal of Agriculture and Rural Development, vol. 20, no. 2, pp. 67-73, 2020.

[6] A. I. Daniel, A. O. Fadaka, A. Gokul, O. O. Bakare, O. Aina, S. Fisher, A. F. Burt, V. Mavumengwana, M. Keyster, and A. Klei, "Biofertilizer: the future of food security and food safety," Microorganisms, vol. 10, no. 6, 2022, Art. no. 1220.

[7] V. C. Tran, V. H. La, M. T. Nguyen, T. G. Nguyen, and T. L. A. Hoang, "Selection of highly active bacterial strains for fixing nitrogen and synthesizing indole-3-acetic acid (IAA) from maize cultivation soil in Thai Nguyen province," Journal of Forestry Science and Technology, vol. 12, no. 6, pp. 003-011, 2023.

[8] A. L. Page, Methods of soil analysis. Part 2. Chemical and microbiological properties, Publisher: American Society of Agronomy, Soil Science Society of America, 1982.

[9] J. Chun, J.-H. Lee, Y. Jung, M. Kim, S. Kim, B. K. Kim, and Y.-W. Lim, "EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences," International Journal of systematic evolutionary microbiology, vol. 57, no. 10, pp. 2259-2261, 2007, doi: 10.1099/ijs.0.64915-0.

[10] S. Kumar, G. Stecher, and K. T. Mega, "Molecular evolutionary genetics analysis version 7.0 for bigger datasets," Molecular Biology Evolution, vol. 33, no. 7, pp. 1870-1874, 2016, doi: 10.1093/molbev/msw054.

[11] LPSN: List of Prokaryotic names with Standing in Nomenclature, “Genus Rhizobium,” 2024. [Online]. Available: https://lpsn.dsmz.de/genus/rhizobium. [Accessed Nov. 2024].

[12] H. P. Browne, S. C. Forster, B. O. Anonye, N. Kumar, B. A. Neville, M. D. Stares, D. Goulding, and T. D. Lawley, “Culturing of “unculturable” human microbiota reveals novel taxa and extensive sporulation,” Nature, vol. 533, pp. 543-546, 2016.

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DOI: https://doi.org/10.34238/tnu-jst.11763

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