CHEMICAL COMPOSITION AND IN VITRO  ANTI-INFLAMMATORY ACTIVITY OF COMPOUNDS ISOLATED FROM PHYLLANTHUS EMBLICA L. FRUITS IN VIETNAM

Vũ Thị Thu Lê; Trần Thị Thùy Dương

doi:10.34238/tnu-jst.12307

CHEMICAL COMPOSITION AND IN VITRO ANTI-INFLAMMATORY ACTIVITY OF COMPOUNDS ISOLATED FROM PHYLLANTHUS EMBLICA L. FRUITS IN VIETNAM

About this article

Received: 14/03/25 Revised: 27/03/25 Published: 28/03/25

Authors

1. Vu Thi Thu Le , TNU - University of Agriculture and Forestry
2. Tran Thi Thuy Duong, TNU - University of Agriculture and Forestry

Abstract

Phyllanthus embrica L., belonging to the genus Phyllanthus of the family Euphorbiaceae, was found and distributed in some countries such as India, Malaysia, Thailand, China. In Vietnam, this species is commonly distributed in some provinces such as Cao Bang, Lang Son, Hoa Binh. In Vietnam, Phyllanthus embrica L. fruit is used to treat diarrhea, sore throat, inflammation, and fever. The studys on Phyllanthus embrica L. fruit in our country is still quite limited and incomplete. In this study, from Phyllanthus embrica L. fruit collected in Hoa Binh, Vietnam, the research isolated and determined the structure of 5 compounds including: Quercetin (1), chlorogenic acid (2), gallic acid (3), protocatechuic acid (4) and kaempferol 3-O-β-D-glucopyranosid (5). The results of biological activity evaluation showed that all five compounds exhibited anti-inflammatory activity in vitro. The two compounds gallic acid (3) and protocatechuic acid (4) exhibited the strongest activity with the ability to inhibit NO production on RAW 264.7 macrophages of 79.44 ± 0.23 (%) and 77.55 ± 0.37 (%) respectively; IC50 values were 25.36 µg/mL and 27.37 µg/mL respectively. The results of this study show the potential of using tamarind fruit in developing products to support the treatment of inflammatory diseases to help care for community health.

Keywords

Phyllanthus embrica L.; Chemical compositions; In vitro anti-inflammatory activity; Phyllanthus; Isolated

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References

[1] T. L. Do, Vietnamese medicinal plants and herbs. Medical Publishing House, 2012, pp. 695-696.

[2] B. P. Gaire and L. Subedi, “Phytochemistry, pharmacology and medicinal properties of Phyllanthus emblica Linn.,” Chinese journal of integrative medicine, pp. 1-8, 2014, doi: 10.1007/s11655-014-1984-2.

[3] R. Chaphalkar, K. G. Apte, Y. Talekar, and S. K. Ojha, “Antioxidants of Phyllanthus emblica L. bark extract provide hepatoprotection against ethanol‐induced hepatic damage: a comparison with silymarin,” Oxidative medicine and cellular longevity, vol. 2017, no. 1, 2017, Art. no. 3876040.

[4] N. T. Duong, N. T. Uyen, P. D. Vinh, N. T. Son, L. V. Ha, and P. T. Thuy, “Hepatoprotective Effect of Standardized Dry Extract from Phyllanthus emblica L. Fruits on Hepatotoxicity Model Induced by Carbon-Tetrachloride in Mice,” J. Med. Materials, vol. 24, no. 2, pp. 108-112, 2019.

[5] K. R. Thilakchand, R. T. Mathai, R. T. Ravi, and M. P. Baliga-Rao, “Hepatoprotective properties of the Indian gooseberry (Emblica officinalis Gaertn): a review,” Food & function, vol. 4, no. 10, pp. 1431-1441, 2013.

[6] S. S. Yadav, M. K. Singh, P. K. Singh, and V. Kumar, “Traditional knowledge to clinical trials: A review on therapeutic actions of Emblica officinalis,” Biomedicine & Pharmacotherapy, vol. 93, pp. 1292-1302, 2017.

[7] Y. J. Zhang, T. Abe, T. Tanaka, C. R. Yang, and I. Kouno, “Two new acylated flavanone glycosides from the leaves and branches of Phyllanthus emblica,” Chemical and pharmaceutical bulletin, vol. 50, no. 6, pp. 841-843, 2002.

[8] N. Abdulhamza, “Study of phytochemical composition and antibacterial activity of Emblica officinalis (Amla) fruit extract” AJVS, vol. 6, pp. 107-113, 2013.

[9] N. M. Cuong, P. N. Khanh, T. T. Huong, V. T. Ha, N. T. Cuc, and T. D. Cuong, “Antioxidant, lipid peroxidation inhibitory activities, and hepatoprotective effect of extracts of Phyllanthus emblica L. fruits,” Vietnam Journal of Science and Technology, vol. 60, no. 3, pp. 410-423, 2022.

[10] V. M. Dirsch, H. Stuppner, and A. M. Vollmar, “The Griess assay: suitable for a bio-guided fractionation of anti-inflammatory plant extracts,” Planta Medica, vol. 64, no. 05, pp. 423-426, 1998.

[11] A M. C. Meena and V. Patni, “Isolation and identification of flavonoid “quercetin” from Citrullus colocynthis (Linn.) Schrad,” Asian J Exp Sci, vol. 22, no. 1, pp. 137-142, 2008.

[12] M. L. Suarez-Quiroz, A. A. Campos, G. V. Alfaro, O. González-Ríos, P. Villeneuve, and M. C. Figueroa-Espinoza, “Isolation of green coffee chlorogenic acids using activated carbon,” Journal of Food Composition and Analysis, vol. 33, no. 1, pp. 55-58, 2014.

[13] J. X. Liu, D. L. Di, and Y. P. Shi, “Diversity of chemical constituents from Saxifraga montana H,” Journal of the Chinese Chemical Society, vol. 55, no. 4, pp. 863-870, 2008.

[14] S. W. Chang, K. H. Kim, I. K. Lee, S. U. Choi, S. Y. Ryu, and K. R. Lee, “Phytochemical constituents of Bistorta manshuriensis,” Natural Product Sciences, vol. 15, no. 4, pp. 234-240, 2009.

[15] J. W. Guo, J. M. Chen, L. J. Lin, and F. Xu, “Five chemical constituents of the ethyl acetate fraction from ethanol extract of semen litchi,” J Med Plants Res, vol. 6, no. 1, pp. 168-170, 2012.

DOI: https://doi.org/10.34238/tnu-jst.12307

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