CHARACTERISTICS OF T1 GENERATION SOYBEAN LINES OVEREXPRESSING THE GmDREB7 GENE

Nguyễn Thu Giang; Nguyễn Thị Hải Yến; Vũ Thị Thu Thủy; Nguyễn Hữu Quân; Chu Hoàng Mậu

doi:10.34238/tnu-jst.13248

CHARACTERISTICS OF T1 GENERATION SOYBEAN LINES OVEREXPRESSING THE GmDREB7 GENE

About this article

Received: 18/07/25 Revised: 14/08/25 Published: 14/08/25

Authors

1. Nguyen Thu Giang, 1) TNU - University of Education, 2) TNU - University of Medicine and Pharmacy
2. Nguyen Thi Hai Yen, Pham Ngoc Thach University of Medicine, Ho Chi Minh City
3. Vu Thi Thu Thuy, TNU - University of Education
4. Nguyen Huu Quan, TNU - University of Education
5. Chu Hoang Mau , TNU - University of Education

Abstract

Soybean (Glycine max (L.) Merrill) is a crop of considerable economic importance. However, its productivity is increasingly threatened by soil salinization, driven by factors such as reduced rainfall, excessive application of chemical fertilizers, and rising sea levels. The DREB (Dehydration-Responsive Element Binding) transcription factor gene subfamily in soybean plays a pivotal role in regulating stress-responsive gene expression under adverse environmental conditions, including drought, cold, and salinity. In this study, transgenic soybean lines overexpressing the GmDREB7 gene were evaluated under greenhouse conditions using salt stress (150–250 mM NaCl) and simulated drought treatments. Key parameters assessed included morphological traits, levels of proline, protein, and lipids, as well as physiological indices associated with stress tolerance. The results demonstrated significantly elevated proline accumulation in all transgenic lines relative to wild-type (WT) plants, with increases ranging from 136.59% to 267.03%, particularly notable in lines TG1-5 and TG1-10. While a minor reduction in plant height was observed in transgenic lines, no significant differences were found in pod number per plant, 100-seed weight, protein, and lipid contents compared to WT. The relative indices for drought and salinity tolerance were highest in TG1-5 and TG1-10, ranging from 11.717 to 20.992 and 17.919 to 18.260, respectively. These findings suggest that overexpression of GmDREB7 enhances proline accumulation and confers improved tolerance to drought and salt stress, offering a promising approach for developing stress-resilient soybean cultivars through genetic engineering.

Keywords

Drought tolerant; Glycine max; GmDREB7; Genetic transformation; Salt tolerant

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DOI: https://doi.org/10.34238/tnu-jst.13248

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