CONSTRUCTION OF VECTOR CARRYING RNAi STRUCTURES CONTAINING CP GENE FRAGMENT  FROM COTTON LEAFROLL DWARF VIRUS (CLRDV)

Ngô Mạnh Dũng; Chu Hoàng Hà

CONSTRUCTION OF VECTOR CARRYING RNAi STRUCTURES CONTAINING CP GENE FRAGMENT FROM COTTON LEAFROLL DWARF VIRUS (CLRDV)

About this article

Received: 04/02/21 Revised: 15/03/21 Published: 06/04/21

Authors

1. Ngo Manh Dung , TNU - University of Education
2. Chu Hoang Ha, Institute of Biotechnology - VAST

Abstract

Cotton is one of the most important fiber plants in many countries over the world; it is a source of fiber for the textile industry and a source of oil production and animal feed from seed. Cotton leafroll dwarf virus (CLRDV) causes great damage to the cotton industry. CLRDV belongs to the family Luteoviridae, genus Polerovirus. In order to prevent CLRDV, the transfer of genes carrying RNAi constructs to create natural antiviral properties for plants is a proven effective measure. In this study, we have successfully designed vector carrying RNAi structure (pGWCLRDV-CPi) contained CP gene fragment from CLRDV. CPi is conservative sequences of CP gene CLRDV have size is 351bp. Strains A.tumefaciens carrying structure pGWCLRDV-Cpi and used as valuable raw materials in creating the transgenic cotton line antiretroviral CLRDV.

Keywords

Cotton; CLRDV; CPi genes; Gene tranfes vector; RNAi

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