IN VITRO SELECTION OF Chrysanthemum × morifolium FOR SALT TOLERANCE

La Việt Hồng; Lê Hoàng Đức; Chu Đức Hà; Cao Phi Bằng; Phùng Thị Hà

doi:10.34238/tnu-jst.4445

IN VITRO SELECTION OF Chrysanthemum × morifolium FOR SALT TOLERANCE

About this article

Received: 02/05/21 Revised: 28/07/21 Published: 31/07/21

Authors

1. La Viet Hong , Hanoi Pedagogical University No2
2. Le Hoang Duc, Institute of Biotechnology - VAST
3. Chu Duc Ha, University of Engineering and Technology - Vietnam National University Hanoi
4. Cao Phi Bang, Hung Vuong University
5. Phung Thi Ha, The Yenlang High school - Hanoi

Abstract

The chrysanthemum is one of the most widely cultivated plants and also one of the most important ornamental plants in the world. In this study, we established a systematic regeneration directly adventitous shoots from the leaf segments of chrysanthemum, then determined the concentration of NaCl as a direct selective factor for the adventitous shoots, and finally analyzed the genetic diversity of some selected clones by ISSR-PCR analysis. The results showed that the most suitable medium for the adventitous shoot regeneration from leaf explants in chrysanthemum was MS, 30 g/l sucrose, 7 g/l agar, pH 5.8, supplemented with 0.5 mg/l BAP. After 5 cultured weeks, the number of shoots per explants, the number of leaves per shoot and the length of shoot were 4.37 (shoots/explant), 4.25 (amount of leaves/shoot) and 1.96 (cm), respectively. The medium for direct selection of salinity-tolerant in vitro shoots was the adventitous shoot regeneration medium supplemented with 100 mM NaCl. The 873 primer [5 '- (GACA)₄ -3'] was suitable for analyzing the genetic relationships of selected chrysanthemum lines. By ISSR-PCR analysis, the following five selective chrysanthemum lines (D1-D5) showed the genetic diversity. These chrysanthemum lines are potential materials for more analysis of salinity tolerance.

Keywords

Chrysanthemum; Selection; Salt tolerance; Chrysanthemum× morifolium; Genetic diversity

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DOI: https://doi.org/10.34238/tnu-jst.4445

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