THE EFFECTS OF ZINGIBER ZERUMBET ETHANOL EXTRACT  ON THE PROLIFERATION, CELL CYCLE, APOPTOSIS AND MIGRATION OF AGS GASTRIC CANCER CELLS

Nguyễn Phú Hùng; Lê Thị Thanh Hương; Mai Văn Linh; Trần Văn Phi

doi:10.34238/tnu-jst.4740

THE EFFECTS OF ZINGIBER ZERUMBET ETHANOL EXTRACT ON THE PROLIFERATION, CELL CYCLE, APOPTOSIS AND MIGRATION OF AGS GASTRIC CANCER CELLS

About this article

Received: 09/07/21 Revised: 27/07/21 Published: 28/07/21

Authors

1. Nguyen Phu Hung , TNU - University of Sciences
2. Le Thi Thanh Huong, TNU - University of Sciences
3. Mai Van Linh, TNU - University of Sciences
4. Tran Van Phi, TNU - University of Sciences

Abstract

Gastric cancer is the most common malignant cance worldwide. Although there have been many advances in the current treatment for gastric cancer, the mortality rate from this cancer is still high, requiring further research to find new drugs and supportive treatment products. In this study, we evaluated the effects of ethanol extract from of Zingiber zerumbet leaves on proliferation, cell cycle, apoptosis by MTT assay, Flow cytometry and cell migration analyses. The results showed that the ethanol extract from the Zingiber zerumbet leaves slowed the increase in the number of AGS cells, with an IC₅₀ value of 2.09 mg/mL, in 48 h of treatment. At a concentration of 0.2 mg/mL, the extract stopped the cell cycle in the G0/G1 phase with cell accumulation in this phase of 74.4% compared with 66.9% in the control sample. Apoptosis rate increased to 14.0% (0.2 mg/mL) and 61.2% compared with 4.4% in the control. Furthermore, the ethanol extract reduced the migratory capacity of AGS cells. The data obtained from this study suggested that Zingiber zerumbet is a potential medicinal plant to fight gastric cancer.

Keywords

Gastric cancer; Zingiber zerumbet; Cell migration; Apoptosis; Cell cycle

Full Text:

PDF (Tiếng Việt)

References

[1] F. Mégraud, E. Bessède, and C. Varon, “Helicobacter pylori infection and gastric carcinoma,” Clin Microbiol Infect, vol. 21, no. 11, pp. 984-990, Nov. 2015, doi: 10.1016/j.cmi.2015.06.004.

[2] H. Sung et al., “Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries,” CA Cancer J Clin, vol. 71, no. 3, pp. 209-249, May 2021, doi: 10.3322/caac.21660.

[3] A. Ohtsu, “Chemotherapy for metastatic gastric cancer: past, present, and future,” J Gastroenterol, vol. 43, no. 4, pp. 256-264, 2008, doi: 10.1007/s00535-008-2177-6.

[4] N. J. Yob, S. M. Jofrry, M. M. R. M. M. Affandi, L. K. Teh, M. Z. Salleh, and Z. A. Zakaria, “Zingiber zerumbet (L.) Smith: A Review of Its Ethnomedicinal, Chemical, and Pharmacological Uses,” Evid Based Complement Alternat Med, vol. 2011, p. 543216, 2011, doi: 10.1155/2011/543216.

[5] Z. A. Zakaria, A. S. Mohamad, C. T. Chear, Y. Y. Wong, D. A. Israf, and M. R. Sulaiman, “Antiinflammatory and antinociceptive activities of Zingiber zerumbet methanol extract in experimental model systems,” Med Princ Pract, vol. 19, no. 4, pp. 287-294, 2010, doi: 10.1159/000312715.

[6] T. Y. Chien, L. G. Chen, C. J. Lee, F. Y. Lee, and C. C. Wang, “Anti-inflammatory constituents of Zingiber zerumbet,” Food Chemistry, vol. 110, no. 3, pp. 584-589, Oct. 2008, doi: 10.1016/j.foodchem.2008.02.038.

[7] K. Kalantari et al., “A Review of the Biomedical Applications of Zerumbone and the Techniques for Its Extraction from Ginger Rhizomes,” Molecules, vol. 22, no. 10, p. E1645, Sep. 2017, doi: 10.3390/molecules22101645.

[8] M. Tian, X. Wu, Y. Hong, H. Wang, G. Deng, and Y. Zhou, “Comparison of Chemical Composition and Bioactivities of Essential Oils from Fresh and Dry Rhizomes of Zingiber zerumbet (L.) Smith,” Biomed Res Int, vol. 2020, p. 9641284, 2020, doi: 10.1155/2020/9641284.

[9] M. H. Zahra et al., “Alpinia zerumbet (Pers.): Food and Medicinal Plant with Potential In Vitro and In Vivo Anti-Cancer Activities,” Molecules, vol. 24, no. 13, p. E2495, Jul. 2019, doi: 10.3390/molecules24132495.

[10] V. C. Vo, Dictionary of Vietnamese medical plants. Medical Publishing House, 2012.

[11] N. T. Nguyen, Research Methods in Plant Sciences. Hanoi National University Publishing House, 2007.

[12] E. A. Asl, J. F. Mehrabadi, D. Afshar, H. Noorbazargan, H. Tahmasebi, and A. Rahimi, “Apoptotic Effects of Linum album Extracts on AGS Human Gastric Adenocarcinoma Cells and ZNF703 Oncogene Expression,” Asian Pac J Cancer Prev, vol. 19, no. 10, pp. 2911-2916, Oct. 2018, doi: 10.22034/APJCP.2018.19.10.2911.

[13] H. S. Choi, H.-S. Seo, J. H. Kim, J.-Y. Um, Y. C. Shin, and S.-G. Ko, “Ethanol extract of Paeonia suffruticosa Andrews (PSE) induced AGS human gastric cancer cell apoptosis via fas-dependent apoptosis and MDM2-p53 pathways,” J Biomed Sci, vol. 19, p. 82, Sep. 2012, doi: 10.1186/1423-0127-19-82.

[14] A. Ghasemzadeh, H. Z. E. Jaafar, A. Rahmat, and M. K. Swamy, “Optimization of microwave-assisted extraction of zerumbone from Zingiber zerumbet L. rhizome and evaluation of antiproliferative activity of optimized extracts,” Chem Cent J., vol. 11, p. 5, 2017, doi: 10.1186/s13065-016-0235-3.

[15] T. Otto and P. Sicinski, “Cell cycle proteins as promising targets in cancer therapy,” Nat Rev Cancer, vol. 17, no. 2, pp. 93-115, Feb. 2017, doi: 10.1038/nrc.2016.138.

[16] M. Akimoto, M. Iizuka, R. Kanematsu, M. Yoshida, and K. Takenaga, “Anticancer Effect of Ginger Extract against Pancreatic Cancer Cells Mainly through Reactive Oxygen Species-Mediated Autotic Cell Death,” PLoS ONE, vol. 10, no. 5, p. e0126605, May 2015, doi: 10.1371/journal.pone.0126605.

[17] N. A. Wani et al., “Reprograming of Glucose Metabolism by Zerumbone Suppresses Hepatocarcinogenesis,” Mol Cancer Res, vol. 16, no. 2, pp. 256-268, Feb. 2018, doi: 10.1158/1541-7786.MCR-17-0304.

[18] S. Elmore, “Apoptosis: A Review of Programmed Cell Death,” Toxicol Pathol, vol. 35, no. 4, pp. 495-516, Jun. 2007, doi: 10.1080/01926230701320337.

[19] I. M. Ghobrial, T. E. Witzig, and A. A. Adjei, “Targeting apoptosis pathways in cancer therapy,” CA Cancer J Clin, vol. 55, no. 3, pp. 178-194, Jun. 2005, doi: 10.3322/canjclin.55.3.178.

[20] S. L. Ham et al., “Phytochemicals potently inhibit migration of metastatic breast cancer cells,” Integr Biol (Camb), vol. 7, no. 7, pp. 792-800, Jul. 2015, doi: 10.1039/c5ib00121h.

[21] M. Rashidi et al., “Selective Cytotoxicity and Apoptosis-Induction of Cyrtopodion scabrum Extract Against Digestive Cancer Cell Lines,” Int J Cancer Manag, vol. 10, no. 5, May 2017, doi: 10.5812/ijcm.8633.

DOI: https://doi.org/10.34238/tnu-jst.4740

Refbacks

There are currently no refbacks.



Remember me