INVESTIGATION OF CHEMICAL COMPOSITIONS AND BIOLOGICAL ACTIVITIES OF WEIGELA FLORIDA “PINK POPPET”

Nguyễn Đức Hùng; Phạm Văn Khang; Từ Quang Tân

doi:10.34238/tnu-jst.5499

INVESTIGATION OF CHEMICAL COMPOSITIONS AND BIOLOGICAL ACTIVITIES OF WEIGELA FLORIDA “PINK POPPET”

About this article

Received: 06/02/22 Revised: 27/04/22 Published: 28/04/22

Authors

1. Nguyen Duc Hung , TNU - University of Education
2. Pham Van Khang, TNU - University of Education
3. Tu Quang Tan, TNU - University of Education

Abstract

In this study, qualitative analysis of ethanolic crude extract from the roots of W. florida “Pink Poppet” was investigated. The results revealed that the presence of flavonoid, terpenoid, coumarin and saponin triterpene, but not for alkaloid, steroid and cardiac glycoside. The antioxidant activity was carried out due to the DPPH free radical scavenging resulting in EC₅₀ value of 81.13 µg/mL. The biological activity of this crude extract was further evaluated on cytotoxicity against mouse colon cancer cells (CT26), mouse melanoma cells (B16) and human liver cancer cells (HepG2) by MTS assay. The results displayed the good cytotoxicities of ethanolic crude extract with IC₅₀ values of 19.25; 9.16; 16.18 µg/mL, in comparison with 5-Fluorouracil as a positive control with IC₅₀values of 30.23, 16.70, 26.19 µg/mL, respectively. These results suggest a further investigation on isolation and evaluation on chemical compounds from the ethanolic crude extract from roots of W. florida “Pink Poppet”.

Keywords

Weigela florida “Pink Poppet”; Antioxidant activity; Biological activity; Chemical composition; Cytotoxicity

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References

[1] H. Sung, J. Ferlay, R. L. Siegel, M. Laversanne, I. Soerjomataram, A. Jemal, and F. Bray, “Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries,” CA. Cancer J. Clin., vol. 71, no. 3, pp. 209-249, May 2021, doi: 10.3322/caac.21660.

[2] A. T. Mbaveng, F. Damen, J. D. Simo Mpetga, M. D. Awouafack, P. Tane, V. Kuete, and T. Efferth, “Cytotoxicity of crude extract and isolated constituents of the Dichrostachys cinerea bark towards multifactorial drug-resistant cancer cells,” Evidence-Based Complement. Altern. Med., vol. 2019, p. 8450158, 2019, doi: 10.1155/2019/8450158.

[3] J. R. Mann, M. G. Backlund, and R. N. DuBois, “Mechanisms of disease: Inflammatory mediators and cancer prevention,” Nat. Clin. Pract. Oncol., vol. 2, no. 4, pp. 202-210, 2005, doi: 10.1038/ncponc0140.

[4] T. Senawong, S. Khaopha, S. Misuna, J. Komaikul, G. Senawong, P. Wongphakham, and S. Yunchalard, “Phenolic acid composition and anticancer activity against human cancer cell lines of the commercially available fermentation products of Houttuynia cordata,” Sci. Asia, vol. 40, no. 1, pp. 420-427, 2014.

[5] M. J. Balunas and A. D. Kinghorn, “Drug discovery from medicinal plants,” Life Sci., vol. 78, no. 5, pp. 431-441, 2005, doi: 10.1016/j.lfs.2005.09.012.

[6] C.-S. Chang, “Flavonoid chemistry of Weigela (Caprifoliaceae) in Korea,” J. Plant Res., vol. 110, no. 2, pp. 275-281, 1997, doi: 10.1007/BF02509316.

[7] P. T. Thuong, B.-S. Min, W. Jin, M. Na, J. Lee, R. Seong, Y.-M. Lee, K. Song, Y. Seong, H.-K. Lee, K. Bae, and S. S. Kang, “Anti-complementary activity of ursane-type triterpenoids from Weigela subsessilis,” Biol. Pharm. Bull., vol. 29, no. 4, pp. 830-833, 2006, doi: 10.1248/bpb.29.830.

[8] H.-J. Lim, E. Y. Jie, I.-S. Park, S.-J. Kim, W. S. Ahn, S.-I. Jeong, S. W. Kim, and C.-H. Jung, “Anti-inflammatory effects of Weigela subsessilis callus extract via suppression of MAPK and NF-κB signaling,” Plants , vol. 10, no. 8, 2021, doi: 10.3390/plants10081635.

[9] M. Na, P. T. Thuong, I. H. Hwang, K. Bae, B. Y. Kim, H. Osada, and J. S. Ahn, “Protein tyrosine phosphatase 1B inhibitory activity of 24-norursane triterpenes isolated from Weigela subsessilis,” Phyther. Res., vol. 24, no. 11, pp. 1716-1719, Nov. 2010, doi: 10.1002/ptr.3203.

[10] N. Andriamisaina, A.-C. Mitaine-Offer, B. Pruvot, J. Chluba, T. Miyamoto, C. Tanaka, and M.-A. Lacaille-Dubois, “Phytochemistry of Weigela x ‘kosteriana variegata’ (Caprifoliaceae),” Nat. Prod. Commun., vol. 13, no. 4, pp. 403-406, Jan. 2018, doi: 10.1177/1934578X1801300406.

[11] D. H. Nguyen, A.-C. Mitaine-Offer, S. Maroso, A.-M. Papini, T. Paululat, P.-S. Bellaye, B. Collin, O. Chambin, and M.-A. Lacaille-Dubois, “Cytotoxic glycosides from the roots of Weigela x ‘Bristol Ruby,” Fitoterapia, vol. 137, p. 104242, Sep. 2019, doi: 10.1016/J.Fitote.2019.104242.

[12] Y. C. Yoo, G. W. Lee, and Y. H. Cho, “Antioxidant and anti-inflammatory effects of extracts from the flowers of Weigela subsessilis on RAW 264.7 macrophages,” J. Life Sci., vol. 26, no. 3, pp. 338–345, Mar. 2016. doi: 10.5352/JLS.2016.26.3.338.

[13] A. N. Sennikov, D. E. Soltis, D. J. Mabberley, J. W. Byng, M. F. Fay, M. J. M. Christenhusz, M. W. Chase, P. F. Stevens, P. S. Soltis, W. S. Judd, and T. A. P. Group, “An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants: APG IV,” Bot. J. Linn. Soc., vol. 181, no. 1, pp. 1-20, Apr. 2016, doi: 10.1111/boj.12385.

[14] L. Traore, Y. Bekro, J. Pirat, and J. A. Mamyrbeva-bekro, “Study of crude extracts from Cassia sieberiana root bark and Khaya grandifoliola trunk bark: Phytochemical screening, quantitative analysis and radical scavenging activity,” Int. J. Curr. Pharm. Res., vol. 7, no. 3 SE-Original Article(s), pp. 22-26, Jul. 2015.

[15] A. Alqethami and A. Y. Aldhebiani, “Medicinal plants used in Jeddah, Saudi Arabia: Phytochemical screening,” Saudi J. Biol. Sci., vol. 28, no. 1, pp. 805-812, 2021, doi: 10.1016/j.sjbs.2020.11.013.

[16] J. Tabart, C. Kevers, J. Pincemail, J.-O. Defraigne, and J. Dommes, “Comparative antioxidant capacities of phenolic compounds measured by various tests,” Food Chem., vol. 113, no. 4, pp. 1226-1233, 2009, doi: 10.1016/j.foodchem.2008.08.013.

[17] D. H. Nguyen, A.-C. Mitaine-Offer, T. Miyamoto, C. Tanaka, P.-S. Bellaye, B. Collin, O. Chambin, and M.-A. Lacaille-Dubois, “Phytochemical analysis of two Weigela florida cultivars, ‘Pink Poppet’ and ‘Jean’s Gold’,” Phytochem. Lett., vol. 37, pp. 85-89, 2020, doi: 10.1016/j.phytol.2020.04.009.

[18] T. Murayama, A. Kasahara, Y. Shiono, and M. Ikeda, “Structure elucidation of a triterpene glycoside isolated from Weigela hortensis,” Nat. Med., vol. 57, no. 5, pp. 181-184, 2003.

[19] A.-S. Champy-Tixier, A.-C. Mitaine-Offer, F. Real Fernández, T. Miyamoto, C. Tanaka, A.-M. Papini, and M.-A. Lacaille-Dubois, “Oleanane-type glycosides from the roots of Weigela florida ‘rumba’ and evaluation of their antibody recognition,” Fitoterapia, vol. 128, pp. 198-203, 2018, doi: 10.1016/j.fitote.2018.04.017.

[20] A. Rezgui, A.-C. Mitaine-Offer, T. Miyamoto, C. Tanaka, S. Delemasure, P. Dutartre, and M.-A. Lacaille-Dubois, “Oleanolic acid and hederagenin glycosides from Weigela stelzneri,” Phytochemistry, vol. 123, pp. 40-47, 2016, doi: 10.1016/j.phytochem.2015.12.016.

[21] K. E. Heim, A. R. Tagliaferro, and D. J. Bobilya, “Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships,” J. Nutr. Biochem., vol. 13, no. 10, pp. 572-584, 2002, doi: 10.1016/S0955-2863(02)00208-5.

[22] A. H. Cory, T. C. Owen, J. A. Barltrop, and J. G. Cory, “Use of an aqueous soluble tetrazolium/formazan assay for cell growth assays in culture,” Cancer Commun., vol. 3, no. 7, pp. 207-212, 1991.

[23] J. M. Nguta, R. Appiah-Opong, A. K. Nyarko, D. Yeboah-Manu, P. G. A. Addo, I. Otchere, and A. Kissi-Twum, “Antimycobacterial and cytotoxic activity of selected medicinal plant extracts,” J. Ethnopharmacol., vol. 182, pp. 10-15, 2016, doi: 10.1016/j.jep.2016.02.010.

[24] J. P. Ferrer, I. C. Zampini, A. S. Cuello, M. Francisco, A. Romero, D. Valdivia, M. Gonzalez, C. Salas, A. S. Lamar, and M. I. Isla, “Cytotoxic compounds from aerial organs of Xanthium strumarium,” Nat. Prod. Commun., vol. 11, no. 3, p. 1934578X1601100313, Mar. 2016, doi: 10.1177/1934578X1601100313.

DOI: https://doi.org/10.34238/tnu-jst.5499

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