STUDY ON RADIOPROTECTION EFFECT FOR HUMAN LYMPHOCYTES AND FIBROBLASTS OF RESVERATROL-ENCAPSULATED LIPOSOME

Nguyễn Minh Hiệp; Phạm Ngọc Duy; Vũ Ngọc Bích Đào; Trần Thanh Mai; Trần Thị Ngọc Mai; Nguyễn Thị Huỳnh Nga

doi:10.34238/tnu-jst.5556

STUDY ON RADIOPROTECTION EFFECT FOR HUMAN LYMPHOCYTES AND FIBROBLASTS OF RESVERATROL-ENCAPSULATED LIPOSOME

About this article

Received: 19/02/22 Revised: 18/04/22 Published: 18/04/22

Authors

1. Nguyen Minh Hiep , Center of Radiation Technology and Biotechnology
2. Pham Ngoc Duy, Center of Radiation Technology and Biotechnology
3. Vu Ngoc Bich Dao, Center of Radiation Technology and Biotechnology
4. Tran Thanh Mai, Center of Radiation Technology and Biotechnology
5. Tran Thi Ngoc Mai, Center of Radiation Technology and Biotechnology
6. Nguyen Thi Huynh Nga, Dalat University

Abstract

This study aims to evaluate radioprotection effect of resveratrol-encapsulated liposomes (Lip-RES). Firstly, Lip-RES was prepared by a combining method of lipid-film hydration method and sonication. Penetration of Lip-RES into human lymphocytes and human fibroblasts (HF cells) was observed by fluorescene microscopy. The results demonstrated that Lip-RES was successfully prepared with a relatively spherical shape, good characteristics (mean particle size of 103.9 nm, polydispersity index of 0.211, zeta potential of -31.9 mV, encapsulation efficiency of 92.31% and payload of 8.47%), and a relatively high stability. Lip-RES was well penetrated into human lymphocytes and HF cells. Under non-irradiated condition, Lip-RES showed a negligible genotoxicity, but exhibited a significant cytotoxicity for lymphocytes and HF cells at RES concentration of more than 10 µg/mL. Under irradiated condition at a dose of 2 Gy, compared to the control, Lip-RES could decrease the percentage of cell death of 16-30% and decrease the number of foci/cell of 10-15%. This indicates the radioprotection effect of Lip-RES for human lymphocytes and fibroblasts. Moreover, at a RES concentration of 2.5 µg/mL, Lip-RES showed a best radioprotection.

Keywords

Fibroblasts; Liposome; Lymphocytes; Radioprotection; Resveratrol

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DOI: https://doi.org/10.34238/tnu-jst.5556

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