ESEARCH ON PROCEDURE THE MICROPROPAGATION  FOR ORCHID SPECIES Brassavola nodosa

Phạm Thị Thu Nhi; Phạm Quỳnh Anh

doi:10.34238/tnu-jst.5758

ESEARCH ON PROCEDURE THE MICROPROPAGATION FOR ORCHID SPECIES Brassavola nodosa

About this article

Received: 28/03/22 Revised: 27/04/22 Published: 28/04/22

Authors

1. Pham Thi Thu Nhi, Research & Development Center for High Technology Agriculture
2. Pham Quynh Anh , Research & Development Center for High Technology Agriculture

Abstract

Brassavola nodosa belongs to the Orchidaceae with flowers that are various in color, shape, and especially fragrance. Brassavola nodosa are being interested in developing planting areas, creating valuable genetic resources for hybridization, and selecting and creating new orchid varieties. In this study, 7-10 cm young shoots of Brassavola nodosa were surface sterilized and then taken 2 cm disinfected apex for rapid regeneration and multiplication. The results showed that MS media supplemented BA 1.5 mg.L^-1and NAA 0.25 mg.L^-1was
suitable for in vitro shoot regeneration, the rate of shoot regeneration reached 97.78%, the number of generated shoots per explants was 16.89. The frequency of submergence and the duration of submergence for rapid shoot multiplication in the RITA® temporary submerged culture system were 6 times/day and 1 minute, repectively. The multiplication coefficient of shoot reached 14.48 times. Knudson C Mineral Medium (1946) was suitable for rooting of in vitro shoots. The survival rate of plants in the nursery period after 4 weeks of planting was 100%.

Keywords

Brassavola nodosa; Apex-shoot; Knudson C (1946); Rhynchostylys gigantean; RITA®

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DOI: https://doi.org/10.34238/tnu-jst.5758

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