CHARACTERISTICS OF rrn16S, trnK-UUU GENES AND PHYLOGENY OF Adinandra megaphylla Hu SPECIES COLLECTED IN LAO CAI PROVINCE, VIETNAM

Phó Thị Thúy Hằng; Nguyễn Thị Thu Ngà; Nguyễn Hữu Quân; Chu Hoàng Mậu

doi:10.34238/tnu-jst.5795

CHARACTERISTICS OF rrn16S, trnK-UUU GENES AND PHYLOGENY OF Adinandra megaphylla Hu SPECIES COLLECTED IN LAO CAI PROVINCE, VIETNAM

About this article

Received: 01/04/22 Revised: 25/04/22 Published: 26/04/22

Authors

1. Pho Thi Thuy Hang, TNU - University of Education; TNU - University of Medicine and Pharmacy
2. Nguyen Thi Thu Nga, TNU - University of Education
3. Nguyen Huu Quan , TNU - University of Education
4. Chu Hoang Mau, TNU - University of Education

Abstract

Adinandra megaphylla Hu is a plant commonly referred to in Vietnamese as “Sum Lá Lớn” or “Hồng đạm Sapa” which belongs to the Adinandra genus. The extracts from the leaves and stems of this species contain several bioactive compounds which present pharmacological activities. The rrn16S and trnK-UUU genes in the chloroplast genome of A. megaphylla Hu were analyzed to determine the genetic relationship among the species collected in Vietnam and Adinandra species published on GenBank. The results show that the rrn16S and trnK-UUU genes in the chloroplast genome of A. megaphylla Hu are 1490 and 2591 nucleotides, respectively. The nucleotide sequences of the rrn16S and trnK-UUU of A. megaphylla Hu in Northern Vietnam were determined to have high similarity with several species of the Adinandra genus, and this plant is most closely related to A. bockiana. A combination of the rrn16S and trnK-UUU genes can be a DNA barcoding candidate for determining the genetic relationship of A. megaphylla Hu. These results are fundamental for further studies on A. megaphylla Hu and other species of the Adinandra genus and contribute to research on the diversity and conservation of genetic resources in Vietnam.

Keywords

Adinandra; rrn16S; trn-UUU; Adinandra megaphylla Hu; DNA barcode

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References

[1] Vietnam Ministry of Science and Technology, Vietnam Red Book, Plants section. Natural Science and Technology Publishing House, Hanoi, 2007.

[2] Y. Chen, G. Chen, X. Fu, and R. H. Liu, “Phytochemical Profiles and Antioxidant Activity of Different Varieties of Adinandra Tea (Adinandra Jack),” Journal of Agricultural and Food Chemistry, vol. 63, pp. 169-176, 2015.

[3] H. Q. Nguyen, T. K. P. Than, and H. M. Chu, “Study on chemical composition and antibacteria activity of extracts total from leaves of Adiandra lienii species,” Proceedings National Biotechnology Conference 2019, pp. 178-182, 2019.

[4] T. N. L. Nguyen, H. Q. Nguyen, T. T. N. Nguyen, T. T. X. Vi, T. D. Sy, T. T. Nguyen, and H. M. Chu, “Antibacterial, Antioxidant and Anti - Cancerous Activities of Adiandra megaphylla Hu Leaf Extracts,” Biosc. Biotech. Res. Comm., vol. 13, pp. 1015-1020, 2020.

[5] T. N. L. Nguyen and H. Q. Nguyen, “Chemical composition and biological activity of extracts total from stem of Adiandra megaphylla Hu collected in lao cai, Vietnam,” TNU Journal of Science and Technology, vol. 226, no.10, pp. 55-61, 2021.

[6] J. W. Kress, K. J. Wurdack, E. A. Zimmer, L. A. Wei, and D. H. Janzen, “Use of DNA barcodes to identify flowering plants,” Proc. Natl. Acad. Sci. USA, vol. 102, pp. 8369-8374, 2005.

[7] H. Ledford, “Botanical identities: DNA barcoding for plants comes a step closer,” Nature, vol. 451, p. 616, 2008.

[8] T. H. Le, N. L. Nguyen, M. M. Bui, H. H. Ha, T. T. H. Huynh, V. H. Nong, V. H. Ha, and T. T. H. Le, “Application of DNA barcodes in identification of ginseng samples in the genus Panax L.,” Vietnam Journal of Biotechnology, vol. 15, no. 1, pp. 63-72, 2017.

[9] T. N. T. Vu and H. M. Chu, “Use of matK DNA barcode for identification of Jewels of Opar (Talinum paniculatum) samples collected at some localities in the Northern of Vietnam,” TNU Journal of Science and Technology, vol. 184, no. 08, pp. 101-106, 2019.

[10] N. T. T. Pham, D. P. Le, K. T. N. Pham, X. Thipphavong, and M. H. Chu, “DNA barcode of matK combined with ITS effectively distinguishes the medicinal plant Stephania brachyandra Diels collected in Laocai, Vietnam,” Journal of Applied Biology & Biotechnology, vol. 9, pp. 63-70, 2021.

[11] T. T. H. Huynh, Q. H. Dao, H. D. Le, and D. T. Nguyen, “Assessment of classification ability for rbcL and trnL barcode in some Stemonaceae samples collected from northern Vietnam,” Journal of Science and Technology of Vietnam, vol. 63, pp. 25-29, 2021.

[12] H. Q. Nguyen and T. T. G. Kieu, “Use of matK DNA barcode to identify Adinandra samples collected at Lao Cai, Vietnam,” TNU Journal of Science and Technology, vol. 197, pp. 205-210, 2015.

[13] H. Q. Nguyen, T. N. L. Nguyen, T. N. Doan, T. T. N. Nguyen, M. H. Pham, T. L. Le, T. D. Sy, H. H. Chu, and H. M. Chu, “Complete chloroplast geneome of novel Adrinandra megaphylla Hu species: molecular structure, comparative and phylogeneetic analysis,” Scientific Reports, vol. 11, no. 11731, 2021, doi: 10.1038/s41598-021-91071-z.

[14] Q. H. Nguyen, L. T. N. Nguyen, N. T. T. Nguyen, T. D. Sy, M. H. Chu, H. H. Chu, L. T. Le, N. T. Doan, H. M. Pham, and G. T. H. Doan, “Adinandra megaphylla chloroplast, complete genome,” 2021. [Online]. Available: https://www.ncbi.nlm.nih.gov/nuccore/MW697901.1.

[15] S. Kumar, G. Stecher, M, Li, C. Knyaz, and K. Tamura, “MEGA X: molecular evolutionary geneetics analysis across computing platforms,” Mol. Biol. Evol., vol. 35, pp. 1547-1549, 2018.

DOI: https://doi.org/10.34238/tnu-jst.5795

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