A STUDY ON THEPRODUCTION OF E2 RECOMBINANT ANTIGEN FROM CLASSICAL SWINE FEVER VIRUS  BY USING THE PICHIA PASTORIS EXPRESSION SYSTEM

Nghiêm Ngọc Minh; Nguyễn Tuấn Hùng; Nguyễn Thị Thùy Linh; Nguyễn Minh Đức; Nguyễn Bảo Trâm; Vũ Minh Thương; Võ Thị Bích Thủy

doi:10.34238/tnu-jst.6388

A STUDY ON THEPRODUCTION OF E2 RECOMBINANT ANTIGEN FROM CLASSICAL SWINE FEVER VIRUS BY USING THE PICHIA PASTORIS EXPRESSION SYSTEM

About this article

Received: 22/08/22 Revised: 16/09/22 Published: 16/09/22

Authors

1. Nghiem Ngoc Minh , Institute of Genome Research – VAST
2. Nguyen Tuan Hung, VETVACO National Veterinary Joint Stock Company (VETVACO.,JSC)
3. Nguyen Thi Thuy Linh, VETVACO National Veterinary Joint Stock Company (VETVACO.,JSC)
4. Nguyen Minh Duc, Institute of Genome Research – VAST
5. Nguyen Bao Tram, Institute of Genome Research – VAST
6. Vu Minh Thuong, Institute of Genome Research – VAST
7. Vo Thi Bich Thuy, Institute of Genome Research – VAST

Abstract

Classical swine fever (CSF) is a viral infectious disease with high morbidity and mortality in pigs, which was a particular concern for farmers. CSF virus (CSFV), the etiological agent of CSF, is icosahedral and enveloped positive-strand RNA virus that belongs to the Pestivirus genus of the Flaviviridae family with one serotype. The E2 glycoprotein of CSFV is a major determinant of virulence and is involved in virus attachment and entry into target cells. It is the most important viral antigen that induces a protective immune response against CSF. In this study, the CFSV E2 protein recombinant antigen was produced in Pichia pastoris (P.pastoris)SMD1168 using the yeast expression system. The result suggests that the E2 protein recombinant of classical swine fever virus has biological activity natural, which was site recognized by antibodies against classical swine fever virus. E2 protein recombinant antigen is also a potential candidate for developing a new generation vaccine against classical swine fever in Vietnam.

Keywords

Classical swine fever; Recombination protein; Pichia pastoris yeast; pGAPZαC vector; Expression systems

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DOI: https://doi.org/10.34238/tnu-jst.6388

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