STUDY ON ANALGESIC AND ANTI-INFLAMMATORY EFFECTS OF ETHYL ACETAT EXTRACT FROM HYPTIS SUAVEOLENS (L.) POIT

Abstract

This study aims to determine the acute toxicity, analgesic and anti-inflammatory effects of the ethyl acetate fraction from the ethanolic extract of Hyptis suaveolens (L.) Poit. Hyptis suaveolens (L.) Poit was extracted in absolute ethanol 70%. Further, the crude extract was partitioned in n-hexane, chloroform, ethyl acetate, aqueous. The acute toxicity experiment was done as per OECD guidelines 423. The study was done on 3 males and 3 females ICR mice which were administered an initial dose of 5000 mg/kg body weight. No mortality and signs of toxicity were observed in two weeks. Analgesic activity was determined using an acetic acid-induced writhing test on adult male mice. Carrageenan-induced paw oedema model was used for anti-inflammatory activity assessment. In both models, mice were pre-treated for 7 days with the fraction at dose 250 mg/kg, 500 mg/kg and diclofenac 5 mg/kg. In the acetic acid-induced writhing model, the fraction exhibited analgesic effect with 36.78%, 45.53% reduction in writhing response at dose of 250 mg /kg and 500 mg/kg body weight compared to the vehicle group. The anti-inflammatory experiment showed that the extract at both doses significantly (p < 0.05) reduced the paw oedema. The ethyl acetat fraction from Hyptis suaveolens is safe and exhibits analgesic and anti-inflammatory activity.