APPLICATION OF IN VITRO CULTURE TECHNOLOGY IN THE CONSERVATION OF HIBICUS SABDARIFFA LINN GENE RESOURCE

Abstract

Hibiscus sabdaiffa Linn is an extremely valuable medicinal plant. Hibiscus is known for its antibacterial, antifungal and antibacterial actions. Other effectiveness of Hibiscus includes enhancing the digestive, limiting the formation of stones in the urinary tract, supporting liver and bile function, reducing cholesterol and triglycerides in the blood, and limiting the formation of kidney stones, obesity due to the accumulation of fat in the blood, protect the vessel wall, antispasmodic, lower blood pressure. Flavonoid and Cyanidin in the calyx of Hibiscus can protect the walls of blood vessels and have antioxidant properties that help prevent cardiovascular disease and cancer. Currently, Hibiscus sabdariffa is rare in nature, therefore preservation and breeding of Hibiscus are necessary. In this paper, we use in vitro culture techniques to preserve the genetic resource of Hibiscus sabdariffa. The results showed that when using the basic MS environment combine with growth stimulants: BAP 1,5 mg/l, NAA 0,3 mg/l, IBA 1,5 mg/l and 2,4-D 2,5 mg/l, that creates the best growth of Hibiscus sabdariffa plants. Using the tissue culture technique, Hibiscus plant has been rapidly multiplied and successfully preserved.