OPTIMIZATION OF THE EXTRACTION CONDITIONS OF POLYPHENOLS FROM AN XOA LEAVES (HELICTERES HIRSUTA LOUR.)

Huỳnh Thị Như Quỳnh; Nguyễn Thị Vân Anh; Hà Hoàng Anh Vĩnh; Đặng Thị Huyền; Vũ Thị Hồng Nhung; Lê Trung Khoảng

doi:10.34238/tnu-jst.11266

OPTIMIZATION OF THE EXTRACTION CONDITIONS OF POLYPHENOLS FROM AN XOA LEAVES (HELICTERES HIRSUTA LOUR.)

About this article

Received: 08/10/24 Revised: 06/02/25 Published: 07/02/25

Authors

1. Huynh Thi Nhu Quynh, Buon Ma Thuot Medical University
2. Nguyen Thi Van Anh, Buon Ma Thuot Medical University
3. Ha Hoang Anh Vinh, Buon Ma Thuot Medical University
4. Dang Thi Huyen, Buon Ma Thuot Medical University
5. Vu Thi Hong Nhung, Buon Ma Thuot Medical University
6. Le Trung Khoang , Buon Ma Thuot Medical University

Abstract

The aim of the study is to investigate the optimal conditions for extracting polyphenols from the leaves of An Xoa (Helicteres hirsuta Lour.). Firstly, the study was conducted with a single variable effect on yield of extract and total polyphenol content. Then, the influencing factors were included in the Box-Behnken design for the optimization (Design Expert 12 software) including: ethanol concentration (30% - 70%), solvent material ratio (10/1 - 20/1) and extraction time (30 - 90 mins). The total polyphenol content in the extract was quantified with Folin-Ciocalteu reagent by spectrophotometry at 765 nm wavelength. The results showed that the optimal conditions were: ethanol concentration of 70%, solvent material ratio of 12.5/1 (ml/g), and extraction time of 90 mins. The experimental values (total polyphenol content = 245.60 ± 3.18 mg GAE, amount of dry extract = 2.66 ± 0.04 g) were not significantly different from the predicted values (total polyphenol content = 244.91 mg GAE, amount of dry extract = 2.67 g).

Keywords

Extraction; Helicteres hirsuta Lour.; An Xoa leaves; Polyphenol; Optimization

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DOI: https://doi.org/10.34238/tnu-jst.11266

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