ISOLATION AND SELECTION OF CELLULOSE DEGRADING BACTERIA FROM DRAGON FRUIT GROWING SOIL

Quách Văn Cao Thi; Nguyễn Lữ Khôi Minh; Nguyễn Trí Yên Chi

doi:10.34238/tnu-jst.11552

ISOLATION AND SELECTION OF CELLULOSE DEGRADING BACTERIA FROM DRAGON FRUIT GROWING SOIL

About this article

Received: 14/11/24 Revised: 18/06/25 Published: 18/06/25

Authors

1. Quach Van Cao Thi, Vinh Long University of Technology Education
2. Nguyen Lu Khoi Minh, Vinh Long University of Technology Education
3. Nguyen Tri Yen Chi , Vinh Long University of Technology Education

Abstract

Discarded dragon fruit branches, which are mainly composed of cellulose, decompose very slowly in nature and pose a potential risk of spreading pathogens from decayed branches. Therefore, this study is carried out by isolating and selecting cellulose-degrading bacteria for the decomposition of discarded dragon fruit branches. Sixteen bacterial strains were originated from dragon fruit- growing soil in Vinh Long province. Using the Congo Red staining method, the findings identified that 10/16 isolated bacterial strains exhibited cellulose-degrading activity, with clear zone diameters ranging from 2.0 ± 0.10 mm to 9.0 ± 0.10 mm. All bacterial strains TL6, TL10, TL12 và TL15 were capable of degrading dragon fruit branches after 15 days on NB and LB media with decomposition efficiency of 15 - 43% and 19 - 38%, respectively. The effects of temperature and pH on the cellulose-degrading activity of isolate TLLH6 were tested and strain TL6 exhibited the strongest cellulose-degrading activity at pH and temperature of 6.5 - 7.5 and 35 – 40 °C, respectively. Strain TL6 was identified as belonging to the Bacillus genus based on morphological and biochemical characteristics along with the results of 16S rRNA gene sequence analysis. These findings offer a crucial foundation for the application of cellulose-degrading bacteria in the treatment of discarded dragon fruit branches.

Keywords

Bacteria; Cellulose; Dragon fruit; Vinh Long province; 16S rRNA gene

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References

[1] R. Al-Qthanin, A. M. M. E. Salih, F. M. A. Alhafidh, S. A. M. Almoghram, G. A. Alshehri, and N. H. Alahmari, "Assessing the suitability of pitaya plant varieties for cultivation in the arid climate of Saudi Arabia," Heliyon, vol. 10, no. 1, 2024, Art. no. e21651.

[2] T. Nguyen, "Do not increase dragon fruit area, prioritize improving quality," (In Vietnamese), 2024. [Online]. Available: https://nongnghiepmoitruong.vn/tri-thuc-nong-dan/khong-tang-dien-tich-thanh-long-uu-tien-nang-chat-luong-d379142.html. [Accessed Nov. 04, 2024].

[3] Minister of Agriculture and Rural Development, Decision No. 4085/QĐ-BNN-TT dated October 27, 2022 to approve a plan on developing key fruit trees by 2025 and 2030, (In Vietnamese), 2022.

[4] T. Bhatia, D. Bose, D. Sharma, and D. Patel, "A Review on Cellulose Degrading Microbes and its Applications," Industrial Biotechnology, vol. 20, no. 1, pp. 26-39, 2024.

[5] R. Datta, "Enzymatic degradation of cellulose in soil: A review," Heliyon, vol. 10, no. 1, 2024, Art. no. e24022.

[6] I. Shareef, S. M. Gopinath, M. Satheesh, and S. X. Christopher, "Isolation and identification of cellulose degrading microbes," International Journal of Web Engineering and Technology, vol. 4, no. 8, pp. 6798-6793, 2015.

[7] N. S. Khoirunnisa, S. Anwar, and D. A. Santosa, "Isolation and selection of cellulolytic bacteria from rice straw for consortium of microbial fuel cell," Biodiversitas, vol. 21, no. 4, pp. 1686-1696, 2020.

[8] H. D. Tran, V. H. Huynh, D. D. Tran, and T. C. Nguyen, "Isolation of cellulose-degrading microbes for rice straw decomposition," (in Vietnamese), Vietnam Journal of Science and Technology, vol. 60, no. 6, pp. 32-36, 2018.

[9] T. T. T. Nguyen, T. L. Nguyen, and T. D. Tran, “Isolation, screening and identification of microorganisms capable of degrading cellulose to microbial organic fertilizer,” (in Vietnamese), Hue University Journal of Science: Agriculture and Rural Development, vol. 127, no. 3A, pp. 117-127, 2018.

[10] T. P. Ngo, H. V. Duong, and N. D. Cao, "Cellulose degrading ability of bacterial strains isolated from gut of termites in Vinh Long province-Vietnam,” Chemical Engineering Transactions, vol. 88, pp. 1315-1320, 2021.

[11] E. Simo, L. Fonseca, C. Uchima, J. Cota, G. H. Goldman, M. Saloheimo, V. Sacon, and M. Siika-aho, "Development of a low-cost cellulase production process using Trichoderma reesei for Brazilian biorefineries," Biotechnol for Biofuels, vol. 10, 2017, Art. no. 30.

[12] Q. H. Dang, and T. T. T. Nguyen, “Isolation, screening and identification of effective microbial strains to make bio-organic fertilizer from organic solid waste,” (in Vietnamese), The University of Danang - Journal of Science and Technology, vol. 20, no. 5, pp. 56-61, 2022.

[13] D. Bhardwaj, M. W. Ansari, R. K. Sahoo, and N. Tuteja, "Biofertilizers function as key player in sustainable agriculture by improving soil fertility, plant tolerance and crop productivity," Microbial cell factories, vol. 13, no. 66, pp. 1-10, 2014.

[14] P. Chaudhary, S. Singh, A. Chaudhary, A. Sharma, and G. Kumar, "Overview of biofertilizers in crop production and stress management for sustainable agriculture," Frontiers in Plant Science, vol. 13, pp. 1-21, 2022.

[15] T. N. T. Nguyen, “Isolation, screening and identification the cellulose degrading bacteria from pitaya clades,” (in Vietnamese), Vietnam Academy of Agricultural Sciences, 2015.

[16] P. Maravi and A. Kumar, "Isolation, screening and identification of cellulolytic bacteria from soil," Biotechnology Journal International, vol. 24, no. 1, pp. 1-8, 2020.

[17] S. A. Bhagat and S. S. Kokitkar, "Isolation and identification of bacteria with cellulose-degrading potential from soil and optimization of cellulase production," Journal of Applied Biology and Biotechnology, vol. 9, no. 6, pp. 154-161, 2021.

[18] H. T. D. Nguyen, T. P. Ngo, T. B. N. Pham, and T. V. Bui, “Determination of carboxymethyl cellulose and cellulose degrading ability of microorganisms isolated from gut of termites collected in Mang Thit district, Vinh Long province, Vietnam," (in Vietnamese), CTU Journal of Science, vol. 58, Special issue on Natural Sciences, pp. 186-191, 2022.

[19] J. G. Holt, N. R. Krieg, P. H. A. Sneath, J. T. Staley, and S. T. Wiliam, Bergey’s manual
of determinative bacteriology, 9th edition, Lippincott William and Wilkins, Baltimore, USA, 1994.

[20] V. P. Q. Vo and N. D. Cao, “Isolation and identification of cellulolytic bacteria,” CTU University Journal of Science, vol. 2011, no. 18a, pp. 177-184, 2011.

[21] H. Heuer, M. Krsek, P. Baker, K. Smalla, and E. M. Wellington, "Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients," Applied and Environmental Microbiology, vol. 63, no. 8, pp. 3233-3241, 1997.

[22] T. Mai, H. H. Nguyen, and N. T. Duong, "Isolation and identification of cellulose degrading bacteria from Holotrichia parallela and Lubricus terrestris," (in Vietnamese), CTU Journal of Science, vol. 2017, no. 50, pp. 81-90, 2017.

DOI: https://doi.org/10.34238/tnu-jst.11552

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