DEVELOPMENT OF AN HPLC METHOD FOR QUANTIFICATION OF NUCIFERIN IN LOWLP-PT DRY EXTRACT

Hà Thanh Hòa; Phạm Quốc Tuấn; Hà Quang Lợi; Trần Thị Vân Anh; Đào Việt Hưng; Ngô Thị Xuân Thịnh; Dương Quốc Toản; Hà Hương Lan; Nguyễn Thu Quỳnh; Nguyễn Thị Minh Diệp

doi:10.34238/tnu-jst.11605

DEVELOPMENT OF AN HPLC METHOD FOR QUANTIFICATION OF NUCIFERIN IN LOWLP-PT DRY EXTRACT

About this article

Received: 25/11/24 Revised: 21/01/25 Published: 22/01/25

Authors

1. Ha Thanh Hoa, Phu Tho College of Medicine and Pharmacy
2. Pham Quoc Tuan, Phu Tho College of Medicine and Pharmacy
3. Ha Quang Loi, Phu Tho College of Medicine and Pharmacy
4. Tran Thi Van Anh, Phu Tho College of Medicine and Pharmacy
5. Dao Viet Hung, Phu Tho College of Medicine and Pharmacy
6. Ngo Thi Xuan Thinh, Phu Tho College of Medicine and Pharmacy
7. Duong Quoc Toan, Phu Tho College of Medicine and Pharmacy
8. Ha Huong Lan, Phu Tho College of Medicine and Pharmacy
9. Nguyen Thu Quynh, TNU - University of Medicine and Pharmacy
10. Nguyen Thi Minh Diep , Phu Tho College of Medicine and Pharmacy

Abstract

Nuciferin is an aporphine alkaloid, the main component of Lotus leaves (Folium Nelumbinis), used as a marker for standardizing LOWLP-PT dry extract. The extraction and quantification method using high-performance liquid chromatography (HPLC) for this dry extract has been developed and validated. The analysis was performed on an HPLC system using a Phenomenex C18 column (250 × 4.6 mm, 5 µm), with the mobile phase consisting of a mixture of acetonitrile: water: triethylamine: glacial acetic acid (27.5: 70.1: 1.63: 0.75 v/v), operated in isocratic mode at a flow rate of 1.2 mL/min, with a sample injection volume of 10 µl and a detection wavelength of 284 nm using a PDA detector. The method has been validated and complies with ICH and AOAC guidelines. Using the developed method, the content of nuciferin in three lots of the investigated formulation was determined to be in the range of 0.171 - 0.194% based on the amount of dried extract.

Keywords

Nuciferin; Folium Nelumbinis; HPLC; Dry extract; LOWLP-PT

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DOI: https://doi.org/10.34238/tnu-jst.11605

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