INVESTIGATION ON VIRUGOCY OF Vibrio parahaemolyticus  CAUSING DISEASES IN WHITE LEG SHRIMP

Nguyễn Thị Biên Thùy; Nguyễn Thị Hạnh; Lê Thị Mây; Trần Văn Tuấn; Đặng Thị Lụa

doi:10.34238/tnu-jst.11819

INVESTIGATION ON VIRUGOCY OF Vibrio parahaemolyticus CAUSING DISEASES IN WHITE LEG SHRIMP

About this article

Received: 06/01/25 Revised: 11/06/25 Published: 11/06/25

Authors

1. Nguyen Thi Bien Thuy , 1) Research Institute for Aquaculture No. 1, 2) University of Science - Vietnam National University, Hanoi
2. Nguyen Thi Hanh, Research Institute for Aquaculture No. 1
3. Le Thi May, Research Institute for Aquaculture No. 1
4. Tran Van Tuan, University of Science - Vietnam National University, Hanoi
5. Dang Thi Lua, Research Institute for Aquaculture No. 1

Abstract

The study investigated the virulence of Vibrio parahaemolyticus bacteria causing disease in whiteleg shrimp as an important scientific basis for further studies on the immune response and resistance of shrimp to V. parahaemolyticus bacteria. The results of the virulence survey of 9 strains of V. parahaemolyticus bacteria isolated from diseased whiteleg shrimp samples identified 4 strains (CAB16002, CAB19003, CAB25001, and CED12020) carrying 2 toxin genes, pirA^vp and pirB^vp. All 4 strains produced exotoxin enzymes hemolysin, phospholipase, and lipase. Of which, the strongest activity of exotoxin enzymes was found in V. parahaemolyticus strain CED12020. The bacterial strain V. parahaemolyticus CED12020 causes disease and death in whiteleg shrimp at cell densities from 10⁴ cfu ml^-1 to 10⁷ cfu ml^-1 with a mortality rate of 14.17-100%. The LD₅₀ value of V. parahaemolyticus CED12020 on whiteleg shrimp (5-6 g/individual) was determined to be 3.0×10⁵ cfu ml^-1.

Keywords

Extracellular Enzyme; Toxin gene; LD50; Whiteleg Shrimp; Vibrio parahaemolyticus

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DOI: https://doi.org/10.34238/tnu-jst.11819

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