SYNTHESIS OF POLYCATECHIN FROM ANCIENT TEA BY-PRODUCTS AND TESTING ITS EFFECT ON RAPID WOUND HEALING

Nguyễn Thành Dương; Phạm Thu Uyên

doi:10.34238/tnu-jst.11937

SYNTHESIS OF POLYCATECHIN FROM ANCIENT TEA BY-PRODUCTS AND TESTING ITS EFFECT ON RAPID WOUND HEALING

About this article

Received: 21/01/25 Revised: 12/06/25 Published: 12/06/25

Authors

1. Nguyen Thanh Duong , 1) Institute of Tropical Technology - Vietnam Academy of Science and Technology, 2) Graduate University of Science and Technology - Vietnam Academy of Science and Technology
2. Pham Thu Uyen, Institute of Tropical Technology - Vietnam Academy of Science and Technology

Abstract

Polycatechin, a polymer synthesized from catechin units, offers numerous advantages over its monomeric form, including enhanced antioxidant activity, improved antibacterial properties, and broader applications in biomedical fields. This study aims to develop a safe and effective biomaterial for wound healing applications by synthesizing polycatechin from ancient tea by-products. Various methods of polycatechin synthesis were explored, and an enzymatic polymerization method using laccase was employed, providing an eco-friendly process with high-purity yield. The synthesized polycatechin was characterized using UV-Vis and NMR spectroscopy, confirming successful polymerization. Furthermore, the polymer demonstrated superior physicochemical stability, maintaining its structural integrity after 72 hours in both water and PBS environments, compared to free catechin. The synthesis efficiency reached 74–88%, depending on the oxidation level of dextran aldehyde. In vitro cytotoxicity tests indicated that Dex-ald-Cat 66-10 exhibited high biocompatibility with normal fibroblast MRC-5 cells up to 800 µg/mL. In addition, polycatechin formulations with optimized Dex-ald/Catechin ratios promoted cell survival and demonstrated great potential for accelerating wound healing. These results highlight polycatechin as a promising candidate for biomedical applications in wound management and tissue engineering.

Keywords

Polycatechin; Enzymatic polymerization; Antioxidant activity; Wound healing; Tissue engineering

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DOI: https://doi.org/10.34238/tnu-jst.11937

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