DOSE, SOURCE, AND TEMPORAL DYNAMICS OF  GENE EXPRESSION IN LPS-INDUCED INFLAMMATORY RESPONSES IN  FISH LEUCOCYTE CELL MODELS

Nguyễn Thị Mai; Vũ Văn Sáng; Amina Moss

doi:10.34238/tnu-jst.12091

DOSE, SOURCE, AND TEMPORAL DYNAMICS OF GENE EXPRESSION IN LPS-INDUCED INFLAMMATORY RESPONSES IN FISH LEUCOCYTE CELL MODELS

About this article

Received: 19/02/25 Revised: 02/12/25 Published: 02/12/25

Authors

1. Nguyen Thi Mai , Vietnam National University of Agriculture
2. Vu Van Sang, VNU University of Science, Vietnam National University, Hanoi
3. Amina Moss, Institute of Aquaculture, University of Stirling, UK

Abstract

Lipopolysaccharides, endotoxins from Gram-negative bacteria, are widely used as immunostimulants to investigate inflammatory responses in fish. This study examined the effects of lipopolysaccharides dose, source, and exposure time on pro- and anti-inflammatory responses gene expression in head kidney leukocytes of common carp Cyprinus carpio. Leukocytes were exposed to lipopolysaccharides at 0, 10, and 100 µg/mL for 4 and 24h using two sources O11 and O26. Cell viability and the expression of inos, tnf-α, il-1, il-6, and il-10 were measured. Results demonstrated a dose-dependent decline in viability, with significant reductions observed at 100 µg/mL. Lipopolysaccharides strongly induced pro-inflammatory cytokines, particularly il-1 which peaked at 4h and remained elevated at higher doses after 24h. The anti-inflammatory cytokines il-10 was also markedly upregulated at 4h, especially at 100 µg/mL. Lipopolysaccharides sources influenced response magnitude, with O11 eliciting stronger inflammation than O26. Overall, LPS triggered dose- and time-dependent cytokine regulation, highlighting a shift from robust pro-inflammatory activation to anti-inflammatory modulation.

Keywords

LPS; Cytokine; Inflammatory response; Cyprinus carpio; Immunostimulant

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DOI: https://doi.org/10.34238/tnu-jst.12091

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