MOLECULAR PHYLOGENY OF VIETNAMESE Paphiopedilum helenae Aver. POPULATIONS | Tuấn | TNU Journal of Science and Technology

MOLECULAR PHYLOGENY OF VIETNAMESE Paphiopedilum helenae Aver. POPULATIONS

About this article

Received: 11/03/25                Revised: 30/06/25                Published: 30/06/25

Authors

1. Tran Minh Tuan, Ba Vi National Park
2. Chu Ngoc Quan, Ba Vi National Park
3. Bui Thuy Lien, Hanoi Pedagogical University 2
4. Nguyen Minh Sang, Kim Xuyen High School
5. Cao Phi Bang, Hung Vuong University
6. Nguyen Sinh Khang, Institute of Ecology and Biological Resources
7. Le Chi Toan Email to author, Hanoi Pedagogical University 2

Abstract


Paphiopedilum helenae, a member of the genus Paphiopedilum, Orchidaceae, is an endangered species. This study used molecular data with 29 newly sequenced samples of P. helenae in Vietnam to investigate its phylogeny. The results of molecular data analysis strongly support that Paphiopedilum is a monophyletic group. Four subgenera were recorded within Paphiopedilum, namely Parvisepalum, Brachypetalum, Megastaminodium and Paphiopedilum. Compared with previous studies, the results of this study suggest that the population of P. helenae was recorded as monophyletic in the section Paphiopedilum. The individuals of the species are concentrated in a group, especially the individuals of P. helenae in Vietnam are not placed together but are mixed with other individuals of China. These results show that the genetics of Paphiopedilum helenae populations in Vietnam and China are highly conservative, and there are genetic variations in this population; however, these current genetic variations are not significant for taxonomic arrangement and phylogeny.

Keywords


Phylogeny; Molecular; Paphiopedilum helenae; Population; Vietnam

References


[1] Z. J. Liu, X. Q. Chen, and P. J. Cribb, “Paphiopedilum,” in Flora of China, vol. 25, Z.Y. Wu, and P. H. Ravenm, Eds, Missouri Botanical Garden Press, St. Louis and Science Press, Beijing, 2009, pp. 33-44.

[2] R. Govaerts, World checklist of selected plant families (Paphiopedilum), the Royal Botanic Gardens, Kew, 2022.

[3] L. V. Averyanov, P. J. Cribb, P. K. Loc, and N. T. Hiep, “Slipper Orchids of Vietnam; with an Introduction to the Flora of Vietnam”, Compass Press Limited, Royal Botanic Gardens, Kew, London, 2003.

[4] L. V. Averyanov, P. J. Cribb, P. K. Loc, and N. T. Hiep, Slipper Orchids of Vietnam; with an Introduction to the Flora of Vietnam, Transport Publishing House, (in Vietnamese), Ha Noi, 2004.

[5] IUCN, “The IUCN Red List of Threatened Species,” 2022. [Online]. Available: https://www.iucnredlist.org/. [Accessed on 27 March 2024]

[6] A. V. Cox, A. M. Pridgeon, V. A. Albert, and M. W. Chase, “Phylogenetics of the slipper orchids (Cypripediodeae: Orchidaceae); Nuclear rDNA sequences,” Plant Systematics and Evolution, vol. 208, pp. 197–223, 1997.

[7] A. Chochai, I. J. Leitch, M. J. Ingrouille, and M. F. Fay, “Molecular phylogenetics of Paphiopedilum (Cypripedioideae; Orchidaceae) based on nuclear ribosomal ITS and plastid sequences,” Botanical Journal of the Linnean Society, vol. 70, no. 2, pp. 176–196, 2012.

[8] M. Górniak, D. L. Szlachetko, A. K. Kowalkowska, J. Bohdanowicz, and C. X. Canh, “Taxonomic placement of Paphiopedilum canhii (Cypripedioideae; Orchidaceae) based on cytological, molecular and micromorphological evidence,” Molecular Phylogenetics & Evolution, vol. 70, pp. 429–441, 2014.

[9] Y. I. Lee, M. C. Chung, K. Sydara et al., “Taxonomic placement of Paphiopedilum rungsuriyanum (Cypripedioideae; Orchidaceae) based on morphological, cytological and molecular analyses,” Bot. Stud., vol. 58, p. 16, 2017.

[10] C. C. Tsai, P. C. Liao, Y. Z. Ko, C. H. Chen, and Y. C. Chiang, “Phylogeny and phylogeny and historical biogeography of Paphiopedilum Pfitzer (Orchidaceae) based on nuclear and plastid DNA,” Frontiers in Plant Science, vol. 11, pp. 1-14, 2020.

[11] H. T. Vu, Q. L. Vu, T. D. Nguyen, N. Tran, T. C. Nguyen, P. N. Luu, D. D. Tran, T. K. Nguyen, and L. Le, “Genetic diversity and identification of Vietnamese Paphiopedilum species using DNA sequences,” Biology, vol. 9, no. 1, pp. 1-18, 2020.

[12] T. H. Y. Nguyen, D. T. Nguyen, X. Q. Ngo, T. P. Do, and H. M. Chu, “A study on the corretation between DNA markers and morphology characteristics for identification of Paphiopedilum helenae Aver. of Vietnam,” TNU Journal of Science and Technology, vol. 227, no. 05, pp. 178-185, 2022.

[13] M. Kearse, R. Moir, A. Wilson, S. Stones-Havas, M. Cheung, S. Sturrock, S. Buxton, A. Cooper, S. Markowitz, C. Duran, T. Thierer, B. Ashton, P. Mentjies, and A. Drummond, “Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data,” Bioinformatics, vol. 28, pp. 1647-1649, 2012.

[14] A. Stamatakis, “RAxML-VI-HPC, maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models,” Bioinformatics, vol. 22, pp. 2688-2690, 2006.

[15] D. Darriba, G. L. Taboada, R. Doallo, and D. Posada, “jModelTest 2: more models, new heuristics and parallel computing,” Nature Methods, vol. 9, p. 772, 2012.

[16] F. Ronquist and J. P. Huelsenbeck, “MrBayes 3, Bayesian phylogenetic inference under mixed models,” Bioinformatics, vol. 19, pp. 1572-1574, 2003.

[17] M. A. Miller, W. Pfeiffer, and T. Schwartz, “Creating the CIPRES Science Gateway for inference of large phylogenetic trees,” Proceedings of the Gateway Computing Environments Workshop (GCE), New Orleans, USA: Institute of Electrical and Electronics Engineers (IEEE), 2010, pp. 1-8.

[18] A. Rambaut and A. J. Drummond, “Tracer,” 2007. [Online]. Available: http://tree.bio.ed.ac.uk/software/tracer/. [Accessed Jan 05, 2021].

[19] V. Manzanilla, A. Kool, N. L. Nguyen, V. H. Nong, T. T. H. Le, and H. De Boer, “Phylogenomics and barcoding of Panax: Toward the identification of ginseng species,” BMC Evolutionary Biology, vol. 18, pp. 1-14, 2018.

[20] C. T. Le, “Molecular phylogeny of Macrosolen (Blume) Rchb. (Loranthaceae) from Vietnam based on molecular data,” TNU Journal of Science and Technology, vol. 227, no. 5, pp. 261-267, 2022.

[21] R. M. Bateman, “Evolution and classification of European orchids: insights from molecular and morphological characters,” Journal Europsischer Orchideen, vol. 33, pp. 33-119, 2001.




DOI: https://doi.org/10.34238/tnu-jst.12265

Refbacks

  • There are currently no refbacks.
TNU Journal of Science and Technology
Rooms 408, 409 - Administration Building - Thai Nguyen University
Tan Thinh Ward - Thai Nguyen City
Phone: (+84) 208 3840 288 - E-mail: jst@tnu.edu.vn
Based on Open Journal Systems
©2018 All Rights Reserved