MOLECULAR MARKERS FOR SPECIES IDENTIFICATION AND pH EFFECTS ON DUCKWEED SAMPLES COLLECTED IN LAM DONG

Hoàng Thị Như Phương; Hoàng Thanh Tùng

doi:10.34238/tnu-jst.12450

MOLECULAR MARKERS FOR SPECIES IDENTIFICATION AND pH EFFECTS ON DUCKWEED SAMPLES COLLECTED IN LAM DONG

About this article

Received: 01/04/25 Revised: 29/06/25 Published: 29/06/25

Authors

1. Hoang Thi Nhu Phuong , Dalat University
2. Hoang Thanh Tung, Dalat University

Abstract

Duckweeds are monocotyledonous plants, small, and grow quickly. Chloroplast coding genes or non-coding regions can identify duckweeds with more accurate results than identification based on morphology. In this study, three duckweed samples from the Spirodela genus and three from the Lemna genus collected in Lam Dong were identified using the genetic markers atpF-atpH and psbK-psbI. The results indicated that the investigated samples were Spirodela polyrhiza and Lemna aequinoctialis. The polymorphism level of SNPs (Single Nucleotide Polymorphisms) was higher in the atpF-atpH region compared to the psbK-psbI region and was also higher in L. aequinoctialis than in S. polyrhiza. Morphological and size variations of the frond were also analyzed, revealing specific differences among samples within the same species. Additionally, the study demonstrated that pH (5,0 - 8,0) significantly affected the growth rate of the samples. For L. aequinoctialis, sample LD1 flourished at pH 8.0, sample LD2 grew most effectively at pH 5.0, and sample LD3 grew quickly at all investigated pH values. Growth rate variations across pH levels were less noticeable for S. polyrhiza, sample LD1 showed the most significant increase in biomass (about 400%), whereas sample LD3 showed the slowest growth (around 200%) after 16 days of cultivation.

Keywords

atpF-atpH; DNA barcode; Lemna aequinoctialis; psbK-psbI; Spirodela polyrhiza

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DOI: https://doi.org/10.34238/tnu-jst.12450

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