PHYTOCHEMICAL COMPOSITION AND BIOLOGICAL ACTIVITIES OF THE LEAVES AND RHIZOMES OF Hornstedtia sanhan M. F. Newman

Đoàn Thị Ái Nghĩa; Nguyễn Đình Quỳnh Phú; Đoàn Quốc Tuấn; Đoàn Ngọc Phương Linh; Phạm Thị Hiền Thư; Nguyễn Như Hà Giang; Nguyễn Thị Sen; Trương Ngọc Anh Thi

doi:10.34238/tnu-jst.12509

PHYTOCHEMICAL COMPOSITION AND BIOLOGICAL ACTIVITIES OF THE LEAVES AND RHIZOMES OF Hornstedtia sanhan M. F. Newman

About this article

Received: 08/04/25 Revised: 30/10/25 Published: 31/10/25

Authors

1. Doan Thi ai Nghia, University of Medicine and Pharmacy - Hue University
2. Nguyen Dinh Quynh Phu , University of Medicine and Pharmacy - Hue University
3. Doan Quoc Tuan, University of Medicine and Pharmacy - Hue University
4. Doan Ngoc Phuong Linh, University of Medicine and Pharmacy - Hue University
5. Pham Thi Hien Thu, University of Medicine and Pharmacy - Hue University
6. Nguyen Nhu Ha Giang, Family Hospital, Danang
7. Nguyen Thi Sen, University of Medicine and Pharmacy - Hue University
8. Truong Ngoc Anh Thi, University of Medicine and Pharmacy - Hue University

Abstract

Hornstedtia sanhan is traditionally used in indigenous medicine and has been studied for its essential oils. This study aimed to evaluate the phytochemical profiles, total phenolic and flavonoid contents and bioactivities of H. sanhan. Organic compound groups were identified using characteristic tests. Total phenolic and flavonoid contents were determined by Folin-Ciocalteu and AlCl₃ methods. Antioxidant activity was assessed via 1,1-diphenyl-2-picrylhydrazyl and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) assays. The sulforhodamine B assay and the inhibition of nitric oxide production in RAW 264.7 cells were used to determine cytotoxicity and anti-inflammatory activities. Phytochemical screening revealed the presence of flavonoids, tannins, and coumarins in H. sanhan. The methanolic leaf extract showed high total phenolic content (227.73 ± 0.35 mg GAE/g) and greater antioxidant and anti-inflammatory activities than the rhizome, with IC₅₀ values of 10.21 ± 0.29, 3.47 ± 0.07 and 50.35 ± 2.01 μg/mL in 1,1-diphenyl-2-picrylhydrazyl, 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and nitric oxide assays, respectively. Both extracts exhibited cytotoxicity with IC₅₀ values between 50.33 and 76.32 μg/mL. These findings provide new insights into the chemical and biological potential of H. sanhan.

Keywords

Hornstedtia sanhan; Total phenolic; Antioxidant; Cytotoxicity; Anti-inflammatory

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DOI: https://doi.org/10.34238/tnu-jst.12509

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