ANTIMICROBIAL ACTIVITY ASSESSMENT OF FATTY ACIDS EXTRACT FROM VIETNAM VIRGIN COCONUT OIL BY LIPASE FROM ASPERGILLUS NIGER IN ANTIBIOTIC-RESISTANCE BACTERIAL TREATMENT

Đoàn Ngọc Trà My; Phan Thị Cúc

doi:10.34238/tnu-jst.12519

ANTIMICROBIAL ACTIVITY ASSESSMENT OF FATTY ACIDS EXTRACT FROM VIETNAM VIRGIN COCONUT OIL BY LIPASE FROM ASPERGILLUS NIGER IN ANTIBIOTIC-RESISTANCE BACTERIAL TREATMENT

About this article

Received: 09/04/25 Revised: 10/08/25 Published: 11/08/25

Authors

1. Doan Ngoc Tra My , The University of Danang - University of Science and Technology
2. Phan Thi Cuc, The University of Danang - University of Science and Technology

Abstract

This study aims to optimize the incubation time and enzyme concentration of lipase fromAspergillus nigerfor the extraction of fatty acids from virgin coconut oil. It alsoevaluates the antibacterial activity of the extracted samples againstStaphylococcus Aureus and Methicillin-ResistantStaphylococcus Aureus using the agar diffusion method. The results show fatty acidsextracted using lipase at an enzyme concentration of 1.5% and an incubation time of 48 hours achieved a hydrolysis degree of 20.88%. These sample demonstrated strong antibacterial activityagainstS. aureus and Methicillin-ResistantStaphylococcus Aureus when compared to chemical methods and the commercialantibiotic Vancomycin. Importantly, the fatty acids extract showed a lower minimum inhibitory concentration against Methicillin-ResistantStaphylococcus Aureus thanS. aureus(0.313% vs 1.25%). These findings suggest that enzymatically extracted fatty acids offer a sustainaible and efective antibacterial alternative, particularly valuable in addressing antibiotic-resistant infection.

Keywords

Virgin coconut oil; Lipase; Aspergillus Niger; Antibiotic resistance; Staphylococcus Aureus

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References

[1] T. T. Ha, T. T. Le, and T. T. T. An, “Curent situation and some factors related to hand eczema in medical staff at 108 Military Central Hospital,” Journal of 108 - Clinical Medicine and Pharmacy, Special Issue: Scientific Conference on the 70th Anniversary of 108 Military Central Hospital, 2021, pp. 126–130.

[2] F. Khorvash, F. Abdi, H. H. Kashani, F. F. Naeini, and T. Narimani, “Staphylococcus aureus in acne pathogenesis: A case-control study,” Am. J. Med. Sci., vol. 4, no. 11, pp. 573–576, 2012, doi: 10.4103/1947-2714.103317.

[3] P. Nandhini et al., "Recent Developments in Methicillin-Resistant Staphylococcus aureus (MRSA) Treatment: A Review," Antibiotics., vol. 11, no. 4, 2022, Art. no. 606, doi: 10.3390/antibiotics11050606..

[4] G. Parfene, V. Horincar, A. K. Tyagi, A. Malik, and G. Bahrim, “Production of medium chain saturated fatty acids with enhanced antimicrobial activity from crude coconut fat by solid state cultivation of Yarrowia lipolytica,” Food Chem., vol. 136, no. 3–4, pp. 1345–1349, 2013, doi: 10.1016/j.foodchem.2012.09.057.

[5] M. Shilling et al., “Antimicrobial effects of virgin coconut oil and its medium-chain fatty acids on clostridium difficile,” J. Med. Food, vol. 16, no. 12, pp. 1079–1085, 2013, doi: 10.1089/jmf.2012.0303.

[6] Z. Fangfang, B. Xinpeng, G. Wei, G. Wang, Z. Shi, and C. Jun, “Effects of virgin coconut oil on the physicochemical, morphological and antibacterial properties of potato starch-based biodegradable films,” Int. J. Food Sci. Technol., vol. 55, no. 1, pp. 192–200, 2020, doi: 10.1111/ijfs.14262.

[7] J. Salimon, B. M. Abdullah, and N. Salih, “Hydrolysis optimization and characterization study of preparing fatty acids from Jatropha curcas seed oil,” Chem. Cent. J., vol. 5, no. 1, pp. 1–9, 2011, doi: 10.1186/1752-153X-5-67.

[8] F. D. Gunstone, The Chemistry of Oils and Fats: Sources, Composition, Properties and Uses. Oxford, UK: Wiley‑Blackwell, 2004.

[9] C. T. Hou and Y. Shimada, “Lipases,” in Encyclopedia of Microbiology, M. Schaechter, Ed., 3rd ed. Oxford, UK: Academic Press, 2009, pp. 229–242, doi: 10.1016/B978-012373944-5.00153-X.

[10] K. E. Jaeger, S. Ransac, B. W. Dijkstra, C. Colson, M. van Heuvel, and O. Misset, “Bacterial lipases,” FEMS Microbiol. Rev., vol. 15, no. 1, pp. 29–63, 1994, doi: 10.1016/0168-6445(94)90025-6.

[11] L. Nini, L. Sarda, L. C. Comeau, E. Boitard, J. P. Dubès, and H. Chahinian, “Lipase-catalysed hydrolysis of short-chain substrates in solution and in emulsion: A kinetic study,” Biochim. Biophys. Acta - Mol. Cell Biol. Lipids, vol. 1534, no. 1, pp. 34–44, 2001, doi: 10.1016/S1388-1981(01)00172-X.

[12] M. Papagianni, “Advances in citric acid fermentation by Aspergillus niger: Biochemical aspects, membrane transport and modeling,” Biotechnol. Adv., vol. 25, no. 3, pp. 244–263, 2007, doi: 10.1016/j.biotechadv.2007.01.002.

[13] F. O. Nitbani, Jumina, D. Siswanta, and E. N. Solikhah, “Isolation and Antibacterial Activity Test of Lauric Acid from Crude Coconut Oil (Cocos nucifera L.),” Procedia Chem., vol. 18, pp. 132–140, 2016, doi: 10.1016/j.proche.2016.01.021.

[14] M. C. P. Zenevicz, A. Jacques, A. F. Furigo, J. V. Oliveira, and D. de Oliveira, “Enzymatic hydrolysis of soybean and waste cooking oils under ultrasound system,” Ind. Crops Prod., vol. 80, pp. 235–241, 2016, doi: 10.1016/j.indcrop.2015.11.031.

[15] M. Elshikh et al., “Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants,” Biotechnol. Lett., vol. 38, no. 6, pp. 1015–1019, 2016, doi: 10.1007/s10529-016-2079-2.

[16] T. A. V. Nguyen, T. D. Le, H. N. Phan, and L. B. Tran, "Hydrolysis Activity of Virgin Coconut Oil Using Lipase from Different Sources," Scientifica (Cairo), vol. 2018, Jan. 14, 2018, Art. no. 9120942, doi: 10.1155/2018/9120942

[17] H. Alexander et al., "The role of bacterial skin infections in atopic dermatitis: expert statement and review from the International Eczema Council Skin Infection Group," Br. J. Dermatol., vol. 182, no. 6, pp. 1331-1342, 2020, doi: 10.1111/bjd.18643.

DOI: https://doi.org/10.34238/tnu-jst.12519

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