RAPID DETECTION OF THE SRI LANKAN CASSAVA MOSAIC VIRUS CAUSING DISEASE FROM CASSAVA STEM IN THE SOUTHEAST PROVINCES OF VIETNAM BY REAL–TIME PCR METHOD

Nguyễn Thành Luân; Nguyễn Thị Ngọc Châm; Phạm Văn Lộc; Trịnh Thị Hương

doi:10.34238/tnu-jst.12577

RAPID DETECTION OF THE SRI LANKAN CASSAVA MOSAIC VIRUS CAUSING DISEASE FROM CASSAVA STEM IN THE SOUTHEAST PROVINCES OF VIETNAM BY REAL–TIME PCR METHOD

About this article

Received: 14/04/25 Revised: 25/08/25 Published: 25/08/25

Authors

1. Nguyen Thanh Luan , Ho Chi Minh City University of Industry and Trade
2. Nguyen Thi Ngoc Cham, Ho Chi Minh City University of Industry and Trade
3. Pham Van Loc, Ho Chi Minh City University of Industry and Trade
4. Trinh Thi Huong, Ho Chi Minh City University of Industry and Trade

Abstract

TheSri Lanka cassava mosaic virus is a harmful pathogen that significantly impacts the yield and qualify of cassava. This virus is characterized by rapid spread and currently there is no specific treatment. In this study, cassava cuttings showing symptoms of virus infection were collected at four locations in the Southeast provinces. The study employed the CTAB - SDS method to extract and collect the ssDNA content of the Sri Lankan cassava mosaic virus. At the same time, the Real-Time PCR method with specific primers (5F/5R) was used to identify the expression of the protein AV1 and quickly determine the viral load in infected samples. The results indicated that the virus was detected after 22.6 cycles (Ct) with a viral concentration of 4.3×10³ IU/ml. This result opens up the prospect of assessing the risk of virus disease from early stages to minimize risks in Vietnam's agricultural production in the future.

Keywords

Sri lankan cassava mosaic; Cassava stem; Real–Time PCR; Protein AV1; Viral loading

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DOI: https://doi.org/10.34238/tnu-jst.12577

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