ISOLATION AND IDENTIFICATION OF Penicillium oxalicum CAUSING LEAF SPOTS DISEASE OF PINEAPPLE IN KIEN GIANG PROVINCE

Nguyễn Thị Trúc Linh; Cao Đỗ Thảo Nhi; Trần Thị Giang

doi:10.34238/tnu-jst.12599

ISOLATION AND IDENTIFICATION OF Penicillium oxalicum CAUSING LEAF SPOTS DISEASE OF PINEAPPLE IN KIEN GIANG PROVINCE

About this article

Received: 16/04/25 Revised: 14/08/25 Published: 14/08/25

Authors

1. Nguyen Thi Truc Linh, 1) Can Tho University, 2) University of Cuu Long
2. Cao Do Thao Nhi, Can Tho University
3. Tran Thi Giang , Can Tho University

Abstract

Pineapple (Ananas comosus) is a fruit not only known for its mild sweetness and pleasant aroma but also for its rich nutritional value, offering numerous health benefits. Despite its resilience, pineapple cultivation faces significant challenges, particularly leaf spot disease caused by fungal pathogens. This disease leads to plant death, severely impacting growth, yield, and fruit quality. This study aimed to isolate and identify the causal agents of leaf spot disease in pineapple samples collected from Tac Cau islet, Kien Giang province, Vietnam. Initial fungal isolation resulted in 12 fungal strains belonging to the genera Penicillium sp., Curvularia sp., Neoscytalidium sp., and Aspergillus sp.. Following Koch’s postulates verification, five fungal strains demonstrated pathogenicity, with strain KG5 exhibiting the highest disease severity (1.63^a± 0.06 cm) and displaying characteristic leaf spot symptoms on pineapple. Molecular identification confirmed that this strain is closely related to Penicillium oxalicum. This study provides important scientific data on the occurrence of the newly identified fungal strain Penicillium oxalicum, which is reported for the first time as the causal agent of leaf spot disease on pineapple in Vietnam.

Keywords

Ananas comosus; Fungal pathogens; Leaf spot disease; Penicillium oxalicum; Pineapple

Full Text:

PDF

References

[1] M. M. Ali, N. Hashim, S. A. Aziz, and O. Lasekan, “Pineapple (Ananas comosus): A comprehensive review of nutritional values, volatile compounds, health benefits, and potential food products,” Food Research International, vol. 137, 2020, Art. no. 109675.

[2] W. Palachum, W. Choorit, and Y. Chisti, “Nutritionally enhanced probioticated whole pineapple juice,” Fermentation, vol. 7, no. 3, 2021, Art. no. 178.

[3] M. F. Hossain, “Nutritional value and medicinal benefits of pineapple,” International Journal of Nutrition and Food Sciences., vol. 4, no. 1, pp. 84-88, 2015.

[4] Ministry of Agriculture and Rural Development of The Socialist Republic of Viet Nam, “The Master Plan for the Development of Key Fruit Crops by 2025 and 2030,” 2024. [Online]. Available: https://iasvn.org/chuyen-muc/De-an-Phat-trien-cay-an-qua-chu-luc-den-nam-2025-va-2030-16565.html. [Accessed Jul. 07, 2024].

[5] W. C. Chung and T. J. Lee, “Fungal diseases of pineapple: Identification and management strategies,” Journal of Tropical Agriculture, vol. 54, no. 2, pp. 112-126, 2017.

[6] R. Horst, Westcott’s Plant Disease Handbook, 7^th ed., Springer Netherlands, 2008.

[7] J. B. Wu, Y. B. He, and R. S. Chen, “First report of leaf spot disease of pineapple caused by Penicillium oxalicum,” Plant Disease, vol. 106, no. 3, 2022, Art. no. 1065.

[8] P. A. Picos-Munoz, R. S. Garcia-Estrada, J. A. Carrillo-Fasio, J. Leon-Felix, and R. Allende-Molar, “First report of blue mold caused by Penicillium oxalicum in tomato (Solanum lycopersicum) in Mexico,” Plant Disease, vol. 95, no. 9, 2011, Art. no. 1195.

[9] Y. Tang, J. F. Li, T. Wang, T. Lin, G. B. Ma, P. K. Zhu, and L. Xu, “First report of Penicillium oxalicum causing blue mold rot of muskmelon (Cucumis melo) in China,” Plant Disease, vol. 104, no. 5, 2020, Art. no. 1550.

[10] J. X. Han, S. L. Zhang, X. C. Shi, D. D. Herrera-Balandrano, S. Y. Wang, and P. Laborda, “First report of Penicillium oxalicum causing leaf blight on maize in China,” Plant Disease, vol. 107, no. 8, 2023, Art. no. 2554.

[11] Z. Zheng, M. Ma, X. Zhu, N. Li, and P. Cao, “First report of Penicillium oxalicum causing postharvest fruit rot on citrus in China,” Plant Disease, vol. 107, no. 4, 2023, Art. no. 1230.

[12] A. Hasan, B. Tabassum, M. Hashim, and N. Khan, “Role of plant growth promoting rhizobacteria (PGPR) as a plant growth enhancer for sustainable agriculture: A review,” Bacteria, vol. 3, no. 2, pp. 59-75, 2024.

[13] M. A. Burtelow, J. D. Merker, and E. J. Baron, “Growth of Histoplasma capsulatum isolates is better on potato dextrose agar with chloramphenicol than on brain heart infusion agar,” Journal de Mycologie Médicale, vol. 19, no. 3, pp. 197-199, 2009.

[14] Y. Liu, Q. Yao, S. Liang, C. Li, X. Chen, and Z. Li, “Identification of the pathogen causing leaf spot in Zinnia elegans and its sensitivity to five fungicides,” Pathogens, vol. 11, no. 12, 2022, Art. no. 1454.

[15] H. Hamdayanty and N. Malinda, “Isolation and identification of pathogen causes brown spot disease on yam tubers (Pachyrhizus erosus) in Indonesia,” IOP Conference Series: Earth and Environmental Science, vol. 575, no. 1, 2020, Art. no. 012157.

[16] N. De Simone, V. Capozzi, M. L. V de Chiara, M. L. Amodio, S. Brahimi, G. Colelli, D. Drider, G. Spano, and P. Russo, “Screening of lactic acid bacteria for the biocontrol of Botrytis cinerea and the potential of Lactiplantibacillus plantarum for eco-friendly preservation of fresh-cut kiwifruit,” Microorganisms, vol. 9, no. 4, 2021, Art. no. 773.

[17] S. Ratnakomala, Fahrurrozi, and Yopi, “Enhancement of cellulase (CMCase) production from marine actinomycetes Streptomyces sp. Bse 7-9: Optimization of fermentation medium by response surface methodology,” In IOP Conference Series: Earth and Environmental Science, vol. 251, 2019, Art. no. 012005.

[18] A. J. Adeleke, S. A. Odunfa, A. Olanbiwonninu, and M. C. Owoseni, “Production of cellulase and pectinase from orange peels by fungi,” Nature and Science, vol. 10, no. 5, pp. 107-112, 2012.

[19] A. H. Mohamed. F. H. Abd El-Megeed, N. M. Hassanein, S. H. Youseif, P. F. Farag, S. A. Saleh, et al., “Native rhizospheric and endophytic fungi as sustainable sources of plant growth promoting traits to improve wheat growth under low nitrogen input,” Journal of Fungi, vol. 8, no. 2, 2022, Art. no. 94.

[20] D. Zhang, L. Liu, and B.S. Chen, “Marine-derived fungi as a valuable resource for amylases activity screening,” Journal of Fungi, vol. 9, no. 7, 2023, Art. no. 736.

[21] K. Karimi, A. Narmani, I. Pertot, and M. Arzanlou, “Rapid and easy modified plate-based screening methods for quantitative and qualitative detection of protease production by fungi,” Acta Phytopathologica et Entomologica Hungarica, vol. 54, no. 1, pp. 1-8, 2019.

[22] O. C. Kwon, W. T. Ju, H. B. Kim, G. B. Sung, and Y. S. Kim, “Isolation and identification of postharvest spoilage fungi from mulberry fruit in Korea,” Korean Journal of Environmental Agriculture, vol. 37, no. 3, pp. 221-228, 2018.

[23] T. J. White, T. D. Bruns, S. Lee, and J. Taylor, “Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics,” in PCR protocols, a guide to methods and applications, M. A. Innis, D. H. Gelfand, J. J. Sninsky, T. J. White, eds., San Diego, California: Academic Press, 1990, pp. 315-322.

[24] Z. Zaeimian and K. B. Fotouhifar, “First report of Neoscytalidium dimidiatum as the causal agent of leaf blight on Clivia miniate,” Scientific Reports, vol. 13, no. 1, 2023, Art. no. 16110.

[25] N. Kiss, M. Homa, P. Manikandan, A. Mythili, K. Krizsán, R. Revathi, M. Varga, T. Papp, C. Vágvölgyi, L. Kredics, and S. Kocsubé, “New species of the genus Curvularia: C. tamilnaduensis and C. coimbatorensis from fungal keratitis cases in South India,” Pathogens, vol. 9, no. 1, 2019, Art. no. 9.

[26] X. Zhang, H. Xi, K. Lin, Z. Liu, Y. Yu, Y. Sun, and J. Zhao, “Aspergillus leaf spot of field bindweed (Convolvulus arvensis L.) caused by Aspergillus niger in China,” Springer Plus, vol. 5, pp. 1-4, 2016.

[27] C. P. Kubicek, T. L. Starr, and N. L. Glass, “Plant cell wall–degrading enzymes and their secretion in plant-pathogenic fungi,” Annual Review of Phytopathology, vol. 52, no. 1, pp. 427-451, 2014.

[28] Z. Daud, M. Z. M. Hatta, A. S. M. Kassim, H. Awang, and A. M. Aripin, “Exploring of agro waste (Pineapple leaf, corn stalk, and napier grass) by chemical composition and morphological study,” BioResources, vol. 9, no. 1, pp. 872-880, 2013.

[29] A. C. Velho, P. Mondino, and M. J. Stadnik, “Extracellular enzymes of Colletotrichum fructicola isolates associated to apple bitter rot and glomerella leaf spot,” Mycology, vol. 9, no. 2, pp. 145-154, 2018.

[30] D. Figaj, P. Ambroziak, T. Przepiora, and J. Skorko-Glonek, “The role of proteases in the virulence of plant pathogenic bacteria,” International Journal of Molecular Sciences, vol. 20, no. 3, 2019, Art. no. 672.

[31] A. Kubátová, M. Hujslová, J. C. Frisvad, M. Chudíčková, and M. Kolařík, “Taxonomic revision of the biotechnologically important species Penicillium oxalicum with the description of two new species from acidic and saline soils,” Mycological Progress, vol. 18, no. 1-2, pp. 215-228, 2019.

[32] H. M. Pham, D. T. Le, L. T. Le, P. T. M. Chu, L. H. Tran, T. T. Pham, H. M. Nguyen, T. T. Luu, H. Hoang, and H. H. Chu, “A highly quality genome sequence of Penicillium oxalicum species isolated from the root of Ixora chinensis in Vietnam,” G3 Genes Genomes Genetics, vol. 13, no. 2, 2023, Art. no. jkac300.

[33] Z. Sapak and S. A. Nusaibah, “Common diseases in pineapple and their management,” in Advances in Tropical Crop Protection, M.-Y. Wong (Ed.), Springer Nature, 2024, pp. 85-104.

DOI: https://doi.org/10.34238/tnu-jst.12599

Refbacks

There are currently no refbacks.



Remember me