RESEARCH ON SELECTING SYMBIOTIC MICROORGANISMS AS STARTER CULTURE SOURCES FOR FERMENTATION OF KOMBUCHA TEA | Quyến | TNU Journal of Science and Technology

RESEARCH ON SELECTING SYMBIOTIC MICROORGANISMS AS STARTER CULTURE SOURCES FOR FERMENTATION OF KOMBUCHA TEA

About this article

Received: 29/05/25                Revised: 30/10/25                Published: 31/10/25

Authors

1. Ha Thi Quyen Email to author, VNU University of Engineering and Technology
2. Chu Duc Ha, VNU University of Engineering and Technology

Abstract


This study aims to select symbiotic microorganisms capable of fermenting sweet tea. From isolated microorganisms, QS1, QS6, and QS7 strains exhibited the best sugar fermentation capability. QA1, QA4, and QA5 strains grew and reduced pH the fastest in culture medium containing a high alcohol concentration. QL2, QL3, and QL5 strains grew and reduced pH the fastest in culture medium. Symbiotic microorganisms consisting of 3 strains belonging to the three above- mentioned groups were created randomly to ferment sweet tea. The results showed that the microorganisms consisting of QS7+QA1+QL3 had the fastest ability to reduce pH and Brix in sweet tea, and the fermented tea water had the highest microbial density even when the pH in the tea water was very low. Strain QS7 was identified as belonging to Sacharomyces cerevisiae, strain QA1 belonging to Komagataeibacter xylinus and strain QL3 belonging to Lactobacillus acidophilus. These strains were stored for developing a starter culture to be easy for fermentation of Kombucha.

Keywords


Kombucha; Fermented tea; Sacharomyces cerevisiae; Komagataeibacter xylinus; Lactobacillus acidophilus

References


[1] R. Jayabalan, R. V. Malbasa, E. S. Loncar, J. S. Vitas, and M. Sathishkumar, “A Review on kombucha tea - microbiology, composition, fermentation, beneficial effects, toxicity, and tea fungus,” Comprehensive Reviews in Food Science and Food Safety, vol. 13, no. 4, pp. 538-550, 2014.

[2] M. Soltani, M. Farshadfar, H. Shirvani, and A. Yaghotipoor, “Evaluation of the antibacterial effect of a beneficial compound based on the probiotic kombucha and honey,” Honeybee Science Journal, vol. 11, no. 21, 2021, doi: 10.22092/hbsj.2021.125166.

[3] R. Malbaša, E. Lončar, and M. Djurić, “Comparison of the products of Kombucha fermentation on sucrose and molasses,” Food Chemistry, vol. 106, no. 3, pp. 1039-1045, 2008, doi: 10.1016/j.foodchem.2007.07.020.

[4] R. V. Malbaša, E. S. Lončar, J. S. Vitas, and J. M. Čanadanović-Brunet, “Influence of starter cultures on the antioxidant activity of kombucha beverage,” Food Chemistry, vol. 127, no. 4, pp. 1727–1731, 2011, doi: 10.1016/j.foodchem.2011.02.048.

[5] R. Jayabalan, P. Subathradevi, S. Marimuthu, M. Sathishkumar, and K. Swaminathan, “Changes in free-radical scavenging ability of kombucha tea during fermentation,” Food Chemistry, vol. 109, no. 1, pp. 227-234, 2008, doi: 10.1016/j.foodchem.2007.12.037.

[6] I. V ̄ına, R. Linde, A. Patetko, and P. Semjonovs, “Glucuronic acid from fermented beverages: Biochemical functions in humans and itsrole in health protection,” International Journal of Recent Research and Applied Studies, vol. 14, pp. 217–230, 2013.

[7] Z. Trivunović, J. Vitas, V. Puškaš, I. Zahović, M. Zeljko, R. Malbaša, and J. Dodić, “Herbal kombucha vinegar: Biotechnological perspective, biological potential and sensory evaluation,” Innovative Food Science & Emerging Technologies, vol. 101, 2025, Art. no. 03955, doi: 10.1016/j.ifset.2025.103955.

[8] B. Vohra1, S. Fazry, F. Sairi, and B. A. Othman, “Effects of Medium Variation and Fermentation Time Towards the pH Level and Ethanol Content of Kombucha,” AIP Conference Proceedings, vol. 2111, 2019, Art. no. 040008, doi: 10.1063/1.5111247.

[9] B. Wang, K. Rutherfurd-Markwick, X. -X. Zhang, and A. N. Mutukumira, “Isolation and characterisation of dominant acetic acid bacteria and yeast isolated from Kombucha samples at point of sale in New Zealand,” Current Research in Food Science, vol. 5, pp. 835–844, 2022.

[10] N. K. Nguyen, N. T. Dong, H. T. Nguyen, and P. H. Le, “Lactic acid bacteria: Promising supplements for enhancing the biological activities of kombucha,” Springerplus, vol. 4, 2015, Art. no. 91.

[11] Z. Yang, F. Zhou, B. Ji, B. Li, Y. Luo, L. Yang, and T. Li, “Symbiosis between microorganisms from kombucha and kefir: Potential significance to the enhancement of kombucha function,” Applied Biochemistry and Biotechnology, vol. 160, pp. 446–455, 2010.

[12] Y. Wang, B. Ji, W. Wu, R. Wang, Z. Yang, D. Zhang, and W. Tian, “Hepatoprotective effects of kombucha tea: Identification of functional strains and quantification of functional components,” Journal of the Science of Food and Agriculture, vol. 94, pp. 265–272, 2014.

[13] S. Chakravorty, S. Bhattacharya, A. Chatzinotas, W. Chakraborty, D. Bhattacharya, and R. Gachhui, “Kombucha tea fermentation: Microbial and biochemical dynamics,” International Journal of Food Microbiology, vol. 220, pp. 63–72, 2016.

[14] T. P. D. Ngo, P. Thanonkeo, and X. P. Huynh, “Screening useful isolated yeasts for ethanol fermentation at high temperature,” International Journal of Applied Science and Technology, vol. 2, no. 4, pp. 65-71, 2012.

[15] K. Reiner, Catalase Test Protocol, American Society for Microbiology, 2010.

[16] D. Laureys, S. J. Britton, and J. De Clippeleer, “Kombucha tea fermentation: A review,” Journal of the American Society of Brewing Chemists, vol. 78, pp. 165–174, 2020.

[17] S. A.Villarreal-Soto, S. Beaufort, J. Bouajila, J. P. Souchard, and P. Taillandier, “Understanding Kombucha Tea Fermentation: A Review,” Journal of Food Science, vol. 83, pp. 580–588, 2018.

[18] Y. Yamada and P. Yukphan, “Genera and species in acetic acid bacteria,” International Journal of Food Microbiology, vol. 125, no. 1, pp. 15-24, 2008, doi: 10.1016/j.ijfoodmicro.2007.11.077.

[19] M. P. Mokoena, C. A. Omatola, and A. O. Olaniran, “Applications of Lactic Acid Bacteria and Their Bacteriocins against Food Spoilage Microorganisms and Foodborne Pathogens,” Molecules, vol. 26, no. 22, 2021, Art. no. 7055, doi: 10.3390/molecules26227055.

[20] T. K. N. Tran and T. H. Nguyen, "Optimizing fermentation conditions for Kombucha tea rich in glucuronic acid from artichokes," (in Vietnamese), HCMUE Journal of Science, vol. 14, no. 6, pp. 193-200, 2017.

[21] C. F. Diguta, G. D. Nitoi, F. Matei, G. Luta, and C. P. Cornea, “The Biotechnological Potential of Pediococcus spp. Isolated from Kombucha Microbial Consortium,” Foods, vol. 9, 2020, Art. no. 1780.

[22] Z. Yang, F. Zhou, B. Ji, B. Li, Y. Luo, L. Yang, and T. Li, “Symbiosis between microorganisms from kombucha and kefir: Potential significance to the enhancement of kombucha function,” Applied Biochemistry and Biotechnology, vol. 160, pp. 446–455, 2010.

[23] T. P. Nguyen and T. H. Nguyen, “Isolation and selection of symbiotic system between yeast and bacteria in fermented kombucha tea to increase glucuronic acid content,” (in Vietnamese), CTU Journal of Science, vol. 54, no. 1B, pp. 13-19, 2018.




DOI: https://doi.org/10.34238/tnu-jst.12917

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