ENHANCED BIOSYNTHESIS OF Β-CAROTENE IN RECOMBINANT ESCHERICHIA COLI HARBORING A PART OF MEVALONATE PATHWAY

Abstract

β-carotene is a carotenoid pigment which has commercial value. In order to improve the biosynthesis of this compound in a recombinant E. coli strain, a part of mevalonate partway was introduced for enhancing metabolic flow to isopentenyldiphosphate, the building block of all carotenoids. Three genes including mvaK1, mvaK2, and mvaDwere cloned from Enterococcus faeciuminsolated in Vietnam and introduced into pET28 vector forming pET28-mvaK1K2D. Subsequently, this recombinant expression vector was transfomed into a recombinant E. coliBL21(DE3) containing pRSET-iEIBY vector encodes for enzymes responsible for biosynthesis of β-carotenefrom IPP. The twovectorswere co-expressedresulted in colonies in yellow – the color of β-carotene. Compared to the E. coli clone withpRSET-iEIBYonly, β-carotenewassynthesizedstronger in the clone with the addition of a part of mevalonatepathway.