SCREENING PROCEDURE FOR TOTAL DNA EXTRACTION TO STUDY YEAST DIVERSITY BASED ON RAPD MOLECULAR MARKER

Nguyễn Mạnh Tuyền; Vũ Minh Châu; Trần Thị Thúy

doi:10.34238/tnu-jst.13211

SCREENING PROCEDURE FOR TOTAL DNA EXTRACTION TO STUDY YEAST DIVERSITY BASED ON RAPD MOLECULAR MARKER

About this article

Received: 11/07/25 Revised: 02/12/25 Published: 03/12/25

Authors

1. Nguyen Manh Tuyen , Hanoi National University of Education
2. Vu Minh Chau, Xuan Phuong highschool
3. Tran Thi Thuy, Hanoi National University of Education

Abstract

The main advantage of DNA molecular markers, such as RAPD, RFLP, and STR, over morphological, physiological, and biochemical markers in diversity studies is their independence from the culture media and living conditions of organisms. However, the successful application of DNA molecular markers is often strongly dependent on the quality of the DNA obtained using the selected total DNA extraction method. In this study, total DNA samples from yeast strains isolated from natural honey and beeswax in Son La, Vietnam, were extracted according to procedures outlined by Lõoke and by da Silva-Filho. Both were compared to the total DNA extraction process (Control) using the commercial kit from Norgen Biotek™. The results indicated that the total DNA samples obtained from the procedure issued by Lõoke and by da Silva-Filho had concentrations ranging from 40 to 3300 ng/μL, which were equal to or higher than those obtained from the control procedure. Furthermore, the quality of these samples was sufficient to ensure successful PCR-RAPD using the primers M13 and (GTG)₅. Storing the total DNA samples extracted by the procedure proposed by Lõoke and by da Silva-Filho for 3 months at -20 ̊C did not adversely affect the PCR-RAPD results. Based on the findings, the Lõoke procedure was recommended for total DNA extraction from yeast for diversity studies due to its convenience, time efficiency, and reduced labor and chemical requirements.

Keywords

DNA extraction; Molecular marker; RAPD; Total DNA; Yeast

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DOI: https://doi.org/10.34238/tnu-jst.13211

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