AN EVALUATION OF THE STABILITY OF MORPHOLOGICAL CHARACTERISTICS AND THE TOTAL AMOUNT OF SAPONOSIDS IN ACHYRANTHES BIDENTATA IN VITRO ROOTS

Abstract

The root of achyranthes bidentata is often used as a medicine. So, after harvesting the roots, we evaluated the stability of morphological characteristics and the total amount of saponosid in the roots of in-vitro type and seed-planted type. By several methods such as studying the morphological characteristics of the root, qualifying saponosid compounds though the reaction with colors: Liebermann - Burchard, qualifying saponosid compounds though the reaction with  1% NaCl solution, extracting and quantifying saponosid substance. Finally, we got the following results: there is no difference in the morphological characteristics of two types (in-vitro and seed-planted). These reactions resulted in the same color. The total amount of saponosid in the root of achyranthes bidentata  in vitro is not different from that of seed-planted (approximately 3.9% weight).