STUDY ON INDENTIFICATION OF BACILLUS SUBTILIS STRAIN LS6

Abstract

Bacillus is a group of bacteria that presents in the majority of microbial products and production enzyme. The strain Bacilus sp. LS6 having high productivity of extracellular multi-enzyme. In this study, we described the result of indentification strain Bacilus sp. LS6 by study of morphological characteristics, biochemical characteristics and sequencing nucleotide of 16S rRNA fragment. The LS6 strain used carbon sources (inositol, maltose, sucrose, glucose, lactose, sorbitol, xylose, starch, cellulose), but could not grow on medium with 7% NaCl. The 554 bp fragment of 16S rRNA gene of the LS6 strain was isolated by PCR reaction and nucleotide sequencing. The sequencing analysis result has showed that the sequence of 16S rRNA gene of the LS6 strain has high homology to those of some representatives of genus Bacillus (99.5-99.8%). Among them, it has the highest homology with that of Bacillus subtilis strain (accession number KT236337). The LS6 strain was named Bacillus subtilis LS6.