Email this article BUILDING THE METHOD OF QUANTIFICATION OF TOTAL SAPONIN IN ROOTS OF POLISCIAS FRUTICOSA WHICH WERE HARVESTED AT THAI NGUYEN BY UV-VIS SPECTROPHOTOMETRY
About this article
Received: 12/08/19 Revised: 08/10/19 Published: 11/10/19Authors
1. Bui Thi Luyen
, University of Medicine and Pharmacy - TNU2. Hoang Thi Cuc, University of Medicine and Pharmacy - TNU
3. Duong Ngoc Nga, University of Medicine and Pharmacy - TNU
4. Nguyen Hong Thai, University of Medicine and Pharmacy - TNU
5. Nguyen Thi Hong Hanh, University of Medicine and Pharmacy - TNU
Abstract
Objective: To establish for content determination of total saponins in the roots of poliscias fruticosa. Methods:Total saponins was determined by UV-VIS spectrophotometry after extracts of the samplehad been coloured. Results: The methods was linear in the range of 5-30 µg/ml (r=0.9987), and the average recovery was 99.775%, RSD was 3.5% (n=6) and repetition rate with RSD= 4.74%. Conclusion:The methods is qualified for the determination of the contents of saponins in herbal medicine poliscias fruticosa.
Keywords
Full Text:
PDF (Tiếng Việt)References
[1]. Nguyễn Thị Ánh Tuyết, Nguyễn Tấn Thiện, Nguyễn Kim Phi Phụng “ Góp phần tìm hiểu hóa học của Đinh lăng”, Tạp chí hóa học tập 43, trang 624-627, 2005.
[2]. Võ Duy Huấn, Satoshi Yamamura, Kazuhiro Ohtani, Ryoji Kasai, Kazuo Yamasaki, Nguyễn Thới Nhâm và Hoàng Minh Châu, Olean Saponin from polyscias fruticose, Phytochemistry, Vol. 47, pp. 451-457, 1998.
[3]. Bộ y tế , Dược điển Việt Nam V, Nhà xuất bản Y học, Hà Nội, 2018.
[4]. Ngô văn Thu , Hóa học saponin, khoa Dược- Trường Đại học Y Dược Thành phố Hồ Chí Minh, trang 109-114, 1990.
[5]. Huahong Wang, Zhezhi Wang, Wubao Guo, “Comparative determination of ursolic acid and oleanolic acid of Macrocarpium officinalis (Sieb. Et Zucc.) Nakai by RP – HPLC”, Industrial crops and product, 28, pp.328-332, 2008.
[6]. Han Benyong, Chen Ying, Ren Ying, Chen Chaoyin “Content determination of total saponins from Opuntia”, An Indian Journal of Bio technology, 10(18), pp. 10400-10404, 2004.
[7]. Chen Q. Zhang, Zhang W, Chen Z, “Identification and quantification of oleanolic acid and ursolic acid in Chinese herbs by liquid chromatography-ion trap mass spectrometry”, Journal of Biomediacal chromatography, 25 (12), pp. 1381 – 1388, 2011.
[8]. Ngô Ứng Long, Cây đinh lăng, Nxb Bộ nông nghiệp, 1986.
[9]. Nguyễn Thị Nguyệt, Võ Xuân Minh, Một số kết quả nghiên cứu về saponin trong đinh lăng”, Tạp chí Dược học, số 3, tr. 15-16, 1992.
Refbacks
- There are currently no refbacks.