Email this article IN VITRO EVALUATION OF ANTIOXIDANT AND ANTIDIABETIC POTENTIAL OF THE EXTRACTS FROM Miliusa velutina LEAVES
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Received: 17/08/19 Revised: 15/09/19 Published: 20/09/19Authors
1. Tran Chi Linh, Can Tho University2. Dai Thi Xuan Trang
, Can Tho University
Abstract
This study evaluated the in vitro inhibitory effects of different extracts of the Miliusa velutina leaf on the activities of diabetes-related enzymes (α-amylase and α-glucosidase) and chemically induced free radicals. The ethanol, n-hexane, dichloromethane, ethyl acetate and aqueous extracts of Miliusa velutina leaves were subjected to a standard enzyme inhibition assay followed by determination of modes of inhibition of the enzymes. The antioxidant activities of the extracts were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), ferric reducing antioxidant power (FRAP), total antioxidant capacity (TAC), reducing power (RP) assays. The results obtained showed that the aqueous extract of the Miliusa velutina leaves optimally inhibited both α-amylase (IC50=260.46±6.61µg/mL) and α-glucosidase (IC50=14.81±0.58 µg/mL). Beside, the aqueous extract displayed the best DPPH, ABTS, TAC, RP and FRAP radical-scavenging ability, respectively. Results demonstrated that Miliusa velutina leaf extracts can represent an important natural source with high antioxidant potential and significant α-amylase and α-glucosidase inhibitory effects.
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[1]. P. M. Sales, P. M. Souza, L. A. Simeoni, P. O. Magalhães and D. Silveira, “α-Amylase inhibitors: A review of raw material and isolated compounds from plant source”, J. Pharm. Pharmaceut. Sci, 15, pp. 141–183, 2012.
[2]. T. Kuzuyaa, T. Kuzuya, S. Nakagawab, et al., “Report of the committee on the classification and diagnostic criteria of diabetes mellitus”, Diabetes Res. Clin. Pr, 55, pp. 65–85, 2002.
[3]. S. Uzma, K. M. Khan, S. Chigurupati, et al., “New hybrid hydrazinyl thiazole substituted chromones: As potential α-amylase inhibitors and radical (DPPH & ABTS) scavengers”, Scientific Reportsvolume, 7, pp. 16980, 2017.
[4]. S. E. Inzucchi, “Oral antihyperglycemic therapy for type 2 diabetes”, Scientific Review and Clinical Applications, 287, pp. 360–372, 2002.
[5]. B. R. Kamesawara, R. Giri, M. M. Kesavulu, C. H. Apparao, “Effect of oral administration of bark extracts of Pterocarpus santalinus L. on blood glucose level in experimental animals”, J Ethnopharmacol, 74, pp. 69–74, 2001.
[6]. M.S. Fageyinbo, A. J. Akindele, S. O. Adenekan, E.O. Agbaje, “Evaluation of in-vitro and in-vivo antidiabetic, antilipidemic and antioxidant potentials of aqueous root extract of Strophanthus hispidus DC (Apocynaceae)”, Journal of Complementary and Integrative Medicine, pp. 1–20, 2019.
[7]. L. M. McCune, T. Jhons, “Antioxidant activity in medicinal plants associated with the symptoms of diabetes mellitus used by the indigenous peoples of the North American boreal forest”, J Ethnopharmacol, 82, pp. 197–205, 2002.
[8]. M. Gul, F. Z. Kutay, S. Temocin, O. Hanninen, “Cellular and clinical implications of glutathione”, Ind J Exp Biol, 38, pp. 625–634, 2000.
[9]. H. Ali, P. J. Houghton, “Alpha amylase inhibitory activity of some Malaysian plants used to treat diabetes, with particular reference to Phyllanthusamarus”, J Ethnopharmacol, 107, pp. 449–455, 2006.
[10]. R. Tundis, M. R. Loizzo, F. Menichini, “Natural products as alpha-amylase and alpha-glucosidase inhibitors and their hypoglycemic potential in the treatment of diabetes: an update”, Mini Rev Med Chem, 10(4), pp. 315–331, 2010.
[11]. T. Chaowasku, J. A. Paul, A. Keßler. “Miliusa cambodgensis sp. nov. (Annonaceae) from Cambodia and M. astiana, M. ninhbinhensis spp. nov. from Vietnam”, Nordic Journal of Botany, 32(3), pp. 297–307, 2014.
[12]. P. Trinop, K. Kanokmedhakula, S. Tontaphab, et al., “Bioactive homogentisic acid derivatives from fruits and flowers of Miliusa velutina”, Fitoterapia, 134, pp. 65–72, 2019.
[13]. N. Wongsa, S. Kanokmedhakul, K. Kanokmedhakul, “Cananginones A-I, linear acetogenins from the stem bark of Cananga latifolia”, Phytochemistry, 72, pp. 1859–1864, 2011.
[14]. N. Wongsa, S. Kanokmedhakul, K. Kanokmedhakul, “Corrigendum to “Cananginones A–I, linear acetogenins from the stem bark of Cananga latifolia”, Phytochemistry, 109: 154, 2015.
[15]. N. Wongsa, K. Kanokmedhakul, J. Boonmak, S. Youngme, S. Kanokmedhakul, “Bicyclic lactones and racemic mixtures of dimeric styrylpyrones from the leaves of Miliusa velutina”, RSC Advances. 7, pp. 25285–25297, 2017.
[16]. Võ Văn Chi, Cây thuốc An Giang, Ủy Ban Khoa học Kỹ thuật An Giang, 1991.
[17]. Nguyễn Kim Phi Phụng, Phương pháp cô lập hợp chất hữu cơ, Nhà xuất bản Đại học Quốc gia Tp. Hồ Chí Minh, 2007.
[18]. P. Prieto, M. Pineda, M. Aguilar, “Spectrophotometric quantification of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E”. Anal Biochem, 269, pp. 337–341, 1999.
[19]. N. Nenadis, , L. F. Wang, M. Tsimidou, H. Y. Zhang, “Estimation of scavenging activity of phenolic compounds using the ABTS•+ assay”, Journal of agricultural and food chemistry, 52, pp. 4669–4674, 2004.
[20]. M. Oyaizu, “Studies on product of browning reaction prepared from glucose amine”, The Japanese Journal of Nutrition and Dietetics. 44, pp. 307–315, 1986.
[21]. O. P. Sharma, T. K. Bhat, “DPPH antioxidant assay revisited”, Food chemistry, 113, pp. 1202–1205, 2009.
[22]. I. F. F.Benzie, J. J. Strain, “The ferric reducing ability of plasma (FRAP) as a measure of ‘antioxidant power’: the FRAP assay”, Analytical Biochem, 239(1), 70–76,1996.
[23]. Đái Thị Xuân Trang, Phạm Thị Lan Anh, Trần Thanh Mến và Bùi Tấn Anh, “Khảo sát khả năng điều trị bệnh tiểu đường của cao chiết lá ổi (Psidium guajava L.). Tạp chí khoa học Đại học Cần Thơ, 22(b), pp. 163–171, 2012.
[24]. L. J. Shai, S. R. Magano, S. L. Lebelo, A. M. Mogale, “Inhibitory effects of five medicinal plants on rat alpha-glucosidase: Comparison with their effects on yeast alpha-glucosidase”, Journal of Medicinal Plants Research, 5, pp. 2863–2867, 2011.
[25]. J. S. Boeing, É. O. Barizão, B. C. e Silva, P. F. Montanher, V. de Cinque Almeida, J. V. Visentainer, “Evaluation of solvent effect on the extraction of phenolic compounds and antioxidant capacities from the berries: application of principal component analysis”, Chemistry Central Journal, 8(1), pp. 48, 2014.
[26]. A. Farahziela, C. N. M. Taib, M. A. M. Moklas, S. M. Akhir, “Antioxidant properties of crude extract, partition extract, and fermented medium of dendrobium sabin flower”, Evidence-Based Complementary and Alternative Medicine, pp. 1–9, 2017.
[27]. C. H. Jao, W. C. Ko, “1, 1- Dipheny1-2 picryl hydrazyl (DPPH) radical scavenging by protein hydrolysates from tuna cooking juice”, Fisheries Science, 68, pp. 430–435, 2002.
[28]. H. Zübeyir, B. Şükrü, İ. Gülçin, “Antioxidant and antiradical properties of selected flavonoids and phenolic compounds. Biochemistry Research International, pp. 1–10, 2017.
[29]. N. Öztaşkin, Y. Çetinkaya, P. Taslimi, S. Göksu, and I. Gülçin, “Antioxidant and acetylcholinesterase inhibition properties of novel bromophenol derivatives”, Bioorganic Chemistry, 60, pp. 49–57, 2015.
[30]. E. De Sousa, L. Zanatta, I. Seifriz, et al., “Hypoglycemic effect and antioxidant potential of kaempferol-3,7-O-(alpha)-dirhamnoside from Bauhinia forficata leaves”, J Nat Prod, 67, pp. 829–832, 2004.
[31]. T. Hanamura, T. Hagiwara, H. Kawagishi, “Structural and functional characterization of polyphenols isolated from acerola (Malpighia emarginata DC.) fruit”, Biosci Biotechnol Biochem, 69, pp. 280–286, 2005.
[32]. E. Thilagam, B. Parimaladevi, C. Kumarappan, S. C. Mandal, “α-Glucosidase and α-amylase inhibitory activity of Senna surattensis”, J Acupunct Meridian Stud, 6, pp. 24–30, 2013.
[33]. L. Hind, N. Benariba, S. Adjdir, R. Djaziri, “In vitro α-amylase and α-glucosidase inhibitory activity of Ononis angustissima extracts. Journal of Applied Pharmaceutical Science, 7(2), pp. 191–198, 2017.
[34]. Nguyen Phuong Thao, Pham Thanh Binh, Nguyen Thi Luyen, Ta Manh Hung, Nguyen Hai Dang, Nguyen Tien Dat, “α-Amylase and α-glucosidase inhibitory activities of chemical constituents from Wedelia chinensis (Osbeck.) Merr. Leaves”, Journal of Analytical Methods in Chemistry, pp. 1–8, 2018.
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