APPLYCATION OF METHYLATED PCR TECHNIQUE TO DETECT METHYLATION OF RASSF1A DAPK GENE IN THE BLOOD SIMPLES OF NASOPHARYLGEAL CARCINOMA PATIENTS

Abstract

Methylation that reduces or does not express the cancer gene Rassf1A and DAPK has been shown in numerous studies on lung cancer. However, in Vietnam the research on methylation of tumor surpress gene have not much yet. Although 70% of human cancer is detected now, the last time and the extra time is very short. This relerve process to methylation of the genes of the genes of the genes in the blood of the package of the nasopharylgeal carcinoma. Cross-sectional descriptive study, non-probability sampling with 9 nasopharyngeal carcinoma and 9 normal patient. Meththylation Specipic PCR as methylation state is released tool: RASSF1A gene found for 100% (9/9) is methylated. The DAPK gene does not detect 100% methylation (9/9) in blood samples of patients with nasopharyngeal carcinoma. Both RASSF1A gene and DAPK gene are not found methyl in the normal blood pattern selected as an argument.