STUDYING QUANTIFICATION OF TRANS CINNAMIC ACID IN CINNAMON TWIG BARK BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Abstract

Objectives: This study aims to develop and validate quantitative methods to appropriate the trans-cinnamic acid content in cinnamon bark collected in Lang Son by high performance liquid chromatography. Method: Cinnamon bark was extracted by soxhlet with methanol solution. The cinnamon bark extract was eluted by HPLC using a reverse-phase column (C8, 150 mm x 4.6 mm, 5 μm) mobile phase containing acetonitrile / acetic acid solution 0.2%, (65: 35 v / v) at a flow rate of 1.0 ml/min, isocratic method and ultraviolet detection at 280 nm. The methods are validated indicators: system suitability, specificity, linearity, accuracy, detection limit, quantitation limit. Results: The HPLC method with selected parameters, pic of trans-cinnamic acid has retention time about 7.4 minutes. The methods have high specificity, suitability of the system with the relative standard deviation (RSD%) of the retention time and the area of the peak in the test was 0.89% and 0.10%; accuracy has a recovery rate of 98.19% with a standard deviation of RSD <2%; repetition rate with RSD = 1.60%. A linear response (r ≈ 1) was observed in the range of 0,1 – 1,0 mg/ ml. The method was appropriately formulated to determine the trans-cinnamic acid in herbal medicine.