MICROPROPAGATION OF RHYNCHOSTYLIS GIGANTEA L. ORCHIRD THROUGH PROTOCORM

Đặng Thị Thanh Mai; Vũ Thị Lan

doi:10.34238/tnu-jst.4467

MICROPROPAGATION OF RHYNCHOSTYLIS GIGANTEA L. ORCHIRD THROUGH PROTOCORM

About this article

Received: 10/05/21 Revised: 12/06/21 Published: 21/06/21

Authors

1. Dang Thi Thanh Mai, TNU - University of Medicine and Pharmacy
2. Vu Thi Lan , TNU - University of Sciences

Abstract

Rhynchostylis gigantea L. Orchid is an endangered tropical epiphytic orchid that is threatened with extinction due to over-collection and the loss of suitable habitats. A efficient protocol for micropropagation of Rhynchostylis gigantea L. was establisted successful through protocorm to conserve and develop this orchid. Culture mediums supplementing growth regulators BAP, NAA and coconut water (CW), potato homogenate (PH) and banana extracts for protocorm induction, biomass multiplication and shoot regeneration was investigated. Results showed that MS medium supplemented with 2.0 mg.l^-1 BAP is suitable for protocorm induction from callus, the rate is 100%. The most suitable protocorm multiplication medium is VW medium added 1.0 mg.l^-1BAP, 0.5 mg.l^-1NAA, 100 ml.l^-1 CW, 40 g.l^-1 PH, 30 g.l^-1 banana extracts. The highest protocorm biomass were gained 4.66 times. VW medium supplemented with 100 ml.l^-1 CW, 40 g.l^-1 PH, 30 g.l^-1 banana extracts (ST2 medium) showed the highest efficiency of PLBs biomass multiplication (17.9 times) and the best shoot regeneration. The most suitable rooting medium was the VW medium supplemented with 1.0 mg.l^-1 NAA, 50 g.l^-1 banana extracts with the highest number of roots/shoot (4.00), the root length was 2.33 cm. This procedure can be applied for large-scale production of Rhynchostylis gigantea L. seedlings.

Keywords

Embryogenic cell; Embryogenic callus; Protocorm; Rhynchostylis gigantea L.; Shoot regeneration

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References

[1] T. D. Dinh, V. D. Dang, and D. Q. Tran, "Effect of The Ecological Regions and Gibberellic Acid (GA3) Application on Growth and Flower Development of Rhynchostylis gigantea (Lindley) Rindley," (In Viettnamese), J. Sci. & Devel., vol. 12, no. 7, pp. 1049-1057, 2014.

[2] V. L. Bui, N. T. H. Phuong, L. T. A. Hong, and K. T. V. Tran, "High frequency shoot regeneration from Rhynchostylis gigantea (orchidaceae) using thin cell layers," Plant Growth Regulation, vol. 28, pp. 179–185, 1999.

[3] P. Pathak, S. Verma, A. Prakash, and K. C. Mahant, "Regeneration competence of an ornamentally important epiphytic orchird, Rhynchostylis gigantea (LINDL.) RIDL. Through leaf segments: A study in vitro.," J. Orchid Soc. India, vol. 31, pp. 97-101, 2017.

[4] J. T. Chen and W. C. Chang, "Efficient plant regeneration through somatic embryogenesis from callus cultures of Oncidium (Orchidaceae)," Plant Sci, vol. 160, pp. 87-93, 2000.

[5] Y. Ishii, T. Takamura, M. Goi, and M. Tanaka, "Callus in duction and somatic embryogenesis of Phalaenopsis," Plant Cell Rep, vol. 17, pp. 446-450, 1998.

[6] K. Tokuhara and M. Mii, "Induction of embryogenic callus and cell suspension culture from shoot tips excised from flower stalk buds of Phalaenopsis (Orchidaceae)," In Vitro Cell Devl Biol, vol. 37, pp. 457-461, 2001.

[7] J. T. Chen and W. C. Chang, "Plant regeneration from callus culture of Cymbidium ensifolium var. misericors," Plant Cell Rep, vol. 17, pp. 251-255, 1998.

[8] Z. Y. Li and L. Xu, "In vitro propagation of white-flower mutant of Rhynchostylis gigantea (Lindl.) Ridl. through immature seed-derived protocorm-like bodies," J Hortic For, vol. 1, no. 6, pp. 093-097, 2009.

[9] R. Suphat, T. Sompong, K. Nattaporn, and K. Soisiri, "Micropropagation of Chang Daeng (Rhynchostylis gigantea var. Sagarik) by embryogenic callus," Songklanakarin J. Sci. Technol., vol. 33, no. 6, pp. 659-663, 2011.

[10] S. M. S. Islam and B. Bhattacharjee, "Plant regeneration through somatic embryogenesis from leaf and root explants of Rhynchostylis retusa (L.) Blume," Appl Biol Res, vol. 17, no. 2, pp. 158-165, 2015.

[11] B. Bakul and S. M. I. Shahinul, "The effect of PGRs on in vitro development of protocorms, regeneration and mass multiplication derived from immature seeds of Rhynchostylis retusa (L.) Blume.," Global J. Biol. Biotech., vol. 4, no. 1, p. 121, 2015.

[12] T. T. H. Phan and V. D. Nguyen, "In vitro propagation of (Rhynchostylisgigantea L. ) orchid”," (In Vietnamses), VNU journal of science: Natural science and technology, vol. 33, no. 1, pp. 48-57, 2017.

[13] V. M. Tran, "Micropagation of Rhynchostylis gigantea orchird by somatic embryogenic cultures," CBU International Conference Proceedings, Prague, Czech Republic, 2019, vol. 7, pp. 969-974.

[14] T. Murashige and F. Skoog, "A revised medium for rapid growth and bio-assays with tobacco tissue cultures," Physiol Plant, vol. 15, pp. 473-497, 1962.

[15] E. F. Vacin and E. W. Went, "Some pH changes in nutrient solutions," Bot Gaz, vol. 110, pp. 605-613, 1949.

[16] G. V. Parab and S. Krishnan, "Rapid in vitro mass multiplication of orchids Aerides maculosa Lindl. and Rhynchostylis retusa (L.) Bl. from immature seeds," Indian Journal of Biotechnology, vol. 11, pp. 288 -294, 2012.

[17] V. T. Bui and T. H. G. Nguyen, "Clonal propagation of Rhynchostylis retusa (L.) Blume from immature seeds by in vitro culture," (In Vietnamese), Vietnam Agricultural Science and Technology Journal, vol. 79, no. 6, pp. 25-29, 2017.

[18] T. T. Dang, N. B. H’Yon, T. T. H. Nguyen, V. K. Dinh, V. D. Nong, T. V. Tran, V. H. Quach, and K. C. Vu, "Micropropagation of Dendrobium heterocarpum Lindl.," (In Vietnamese), Journal of Biotechnology, vol. 16, pp. 127-135, 2018.x

DOI: https://doi.org/10.34238/tnu-jst.4467

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