STUDYING THE EFFECT OF A COMBINATION OF PLANT GROWTH REGULATOR ON MULTIPLE SHOOTS AND ROOTING  IN GmCHI TRANSGENIC T. PANICULATUM

Vũ Thị Như Trang; Chu Hoàng Mậu; Ngô Thị Thúy Ngân

doi:10.34238/tnu-jst.4559

STUDYING THE EFFECT OF A COMBINATION OF PLANT GROWTH REGULATOR ON MULTIPLE SHOOTS AND ROOTING IN GmCHI TRANSGENIC T. PANICULATUM

About this article

Received: 28/05/21 Revised: 09/08/21 Published: 13/09/21

Authors

1. Vu Thi Nhu Trang , TNU - University of Medicine and Pharmacy
2. Chu Hoang Mau, TNU - University of Education
3. Ngo Thi Thuy Ngan, TNU - University of Medicine and Pharmacy

Abstract

Talinum paniculatum is a herbaceous plant known for its high medicinal value. GmCHI transgenic T. paniculatum in the T1 generation, that contain 4.24 mg/g and 2.74 mg/g of flavonoid, respectively, which reflect increases of 7.4-fold and 4.8-fold, respectively, compared to that in wild-type plants. In order to increase flavonoid acquisition from the biomass of GmCHI transgenic T. paniculatum in the T1 generation with high flavonoid content, it is necessary to study the effect of multiple shoots and rooting in transgenic plants. In this paper, we present the results of studying the effects of a combination of plant growth regulator on multiple shoots and rooting in GmCHI transgenic T. paniculatum. The cotyledons are suitable materials to create multiple shoot for GmCHI transgenic T. paniculatum when using the MS medium supplemented with kinetin. The addition of 2.0 mg/l of BAP gave a higher multi-shooting effect than the addition of 1.5 mg/l of kinetin or the combination of 1.5 mg/l of kinetin with 0.8 mg/l of IBA. Using 0.5mg/l IAA had better rooting ability than combining 0.5mg/l IAA with kinetin.

Keywords

Culture medium; Multiple shoots; GmCHI transgenic T. paniculatum; The cotyledons; Plant growth regulator

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References

[1] T. Catthareeya, P. Papirom, S. Chanlun, and S. Kupittayanant, “Talinum paniculatum (Jacq.) Gertn: A medicinal plant with potential estrogenic activity in ovariectomized rats,” Int J Pharm Pharm Sci, vol. 5, pp. 478-485, 2013.

[2] Y. S. W. Manuhara, A. Yachya, and A. N. Kristanti, “Effect of aeration and inoculum density on biomass and saponin content of Talinum paniculatum Gaertn. hairy roots in balloon-type bubble bioreactor,” J Pharm Biomed Sci, vol. 2, no. 4, pp. 47-52, 2012.

[3] T. L. Do, Vietnamese medicinal plants and herbs. Medical publishing house, Ha Noi, 2004.

[4] K. Shashank and K. P. Abhay, “Chemistry and biological activities of flavonoid: An overview,” The Scientific World Journal, 2013, Art. no. 162750.

[5] T. N. T. Vu, T. H. T. Le, P. H. Hoang, D. T. Sy, T. T. T. Vu, and H. M. Chu, “Overexpression of the Glycine max chalcone isomerase (GmCHI) gene in transgenic Talinum paniculatum plants,” Turk J Bot, vol. 42, pp. 551-558, 2018.

[6] T. N. T. Vu, T. T. Nguyen, and H. M. Chu, “The develoment in vitro regeneration system for gene transfer in Talinum paniculatum plants,” TNU Journal of Science and Technology, vol. 161, no. 01, pp. 73-79, 2017.[7] H. M. Chu, Modern methods of genetic integration in plant breeding. Thai Nguyen university publishing house, 2008.

DOI: https://doi.org/10.34238/tnu-jst.4559

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