STUDY ON EXPRESSION ENCHANCING OF MIRACULIN  IN VIETNAM TOMATO CULTIVAR

La Việt Hồng; Bùi Phương Thảo; Phạm Bích Ngọc; Chu Hoàng Hà

doi:10.34238/tnu-jst.4923

STUDY ON EXPRESSION ENCHANCING OF MIRACULIN IN VIETNAM TOMATO CULTIVAR

About this article

Received: 25/08/21 Revised: 30/10/21 Published: 30/10/21

Authors

1. La Viet Hong , Institute of Biotechnology - Vietnam Academy of Science and Technology; Hanoi Pedagogical University 2
2. Bui Phuong Thao, Institute of Biotechnology - Vietnam Academy of Science and Technology
3. Pham Bich Ngoc, Institute of Biotechnology - Vietnam Academy of Science and Technology
4. Chu Hoang Ha, Institute of Biotechnology - Vietnam Academy of Science and Technology

Abstract

Miraculin is a natural sweet-tasting protein and has the ability to convert sour taste into sweet taste even though its property is not sweet. In this study, the genetic code of miraculin was optimized. In addition, to enhance the expression of miraculin in transgenic tomato, the fruit-specific E8 promoter from tomato and the HSP terminator from A.thaliana were also used to control the activity of synthetic miraculin. Applying PCR analysis, the results showed that there were 16 transgenic lines carrying E8-pro:Mir:HSP-ter and were 11 transgenic tomato lines carrying transferred miraculin gene, the transgenic efficiency were 42.11% and 52.38%, respectively. Among them, five of these 27 transgenic tomato lines contained a single copy of the targeted gene (T.E8.4, T.E8.7, T.E8.8, T.35S.2, T.35S8). The content of recombinant miraculin tomato lines carrying E8 promoter and terminator HSP 18.2 was higher than the content of miraculin in tomato lines carrying 35S promoter and terminator NOS. The content of recombinant miraculin was highest in the T.E8.8 line, reached 118.0 ng/µg of total soluble protein (equivalent to 11.8% of total soluble protein) and was 4 times higher than that of the T.35S.2, T.35S8.

Keywords

Cultivar; Expression; Enchancing; Miraculin; Promoter; Recombination

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DOI: https://doi.org/10.34238/tnu-jst.4923

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