MORPHOLOGICAL CHARACTERISTICS AND DNA trnH-psbA GENE SEQUENCE IN THE TAXONOMY OF P. hirsutissimum

Nguyễn Thị Hải Yến; Ngô Xuân Quảng; Nguyễn Đình Trọng; Đỗ Tiến Phát

doi:10.34238/tnu-jst.4958

MORPHOLOGICAL CHARACTERISTICS AND DNA trnH-psbA GENE SEQUENCE IN THE TAXONOMY OF P. hirsutissimum

About this article

Received: 08/09/21 Revised: 19/10/21 Published: 25/10/21

Authors

1. Nguyen Thi Hai Yen , TNU - University of Sciences
2. Ngo Xuan Quang, VAST - Institute of Tropical Biology; VAST - Institute of Biotechnology
3. Nguyen Dinh Trong, VAST - Institute of Biotechnology
4. Do Tien Phat, VAST - Institute of Biotechnology; VAST - Graduate University of Sciences and Technology

Abstract

The Paphiopedilum is a small genus with about 80 species but stands out in the evolution of the family Orchidaceae. This genus is also quite special because of the complex taxonomy system with many subgenera, subdivisions and different taxonomic views. The advent of DNA barcodes has greatly supported taxonomic studies beyond traditional morphology-based methods. This paper presents the results of morphological analysis of plant materials of the Venu orchid species collected in Thai Nguyen province, combined with the trnH-psbA gene sequences to identify to species. The results found that the trnH-psbA gene sequence from the researched Venu orchid species samples obtained a gene fragment of 612 bp in size, nucleotide similarity reached 99.34% compared with the trnH-psbA sequence of species P. hirsutissimum (code. MN153815.1) on the Genbank. The taxonomic tree was established based on the trnH-psbA sequence of Venu orchid species with 19 sequences on the NCBI Genbank, showing that trnH-psbA sequence is a potential indicator in the identification and classification of The Paphiopedilum. The branches on the taxonomic tree clearly reflect the relationship of these species which are grouped to subgenera, subdivision much better than that in comparison with previous morphological taxonomy.

Keywords

Taxonomy; trnH-psbA; P. hirsutissimum; Barcode; Thai Nguyen

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DOI: https://doi.org/10.34238/tnu-jst.4958

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