QUALITATIVE ANALYSIS AND EVALUATION BIOLOGICAL ACTIVITIES OF WEIGELA X “BRISTOL RUBY”

Nguyễn Đức Hùng; Lê Phương Dung

doi:10.34238/tnu-jst.5168

QUALITATIVE ANALYSIS AND EVALUATION BIOLOGICAL ACTIVITIES OF WEIGELA X “BRISTOL RUBY”

About this article

Received: 14/10/21 Revised: 31/12/21 Published: 12/01/22

Authors

1. Nguyen Duc Hung , TNU - University of Education
2. Le Phuong Dung, TNU - University of Education

Abstract

In this study, qualitative analysis and biological activities of the ethanolic crude extract from roots of W. x “Bristol ruby” were investigated. The qualitative results showed the presence of flavonoid, terpenoid, coumarin and saponin triterpene in ethanolic crude extract without alkaloid, steroid and cardiac glycoside. The antioxidant activity on this crude extract was revealed with EC₅₀ value of 71.94 µg/mL. The cytotoxic activity of ethanolic crude extract from roots of W. x “Bristol ruby” was further tested against colon cancer cell lines CT26 resulting a good cytotoxicity with an IC₅₀ of 11.83 µg/mL, comparing to 5-Fluorouracil with an IC₅₀ of 36.76 µg/mL. These results could be used further in order to isolate chemical compounds of ethanolic crude extract from roots of W. x “Bristol ruby” and further evaluate those biological activities.

Keywords

Weigela x “Bristol ruby”; Chemical compositions; Antioxidant activity; Cytotoxicity; CT26

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References

[1] H. Sung, J. Ferlay, R. L. Siegel, M. Laversanne, I. Soerjomataram, A. Jemal, and F. Bray, “Global Cancer Statistics 2020: GLOBOCAN Estimates of incidence and mortality worldwide for 36 cancers in 185 countries,” CA. Cancer J. Clin., vol. 71, no. 3, pp. 209-249, 2021, doi: 10.3322/caac.21660.

[2] R. M. Goldberg, D. J. Sargent, R. F. Morton, C. S. Fuchs, R. K. Ramanathan, S. K. Williamson, B. P. Findlay, H. C. Pitot, and S. R. Alberts, “A randomized controlled trial of fluorouracil plus leucovorin, irinotecan, and oxaliplatin combinations in patients with previously untreated metastatic colorectal cancer,” J. Clin. Oncol., vol. 22, no. 1, pp. 23-30, 2004, doi: 10.1200/JCO.2004.09.046.

[3] A. Jemal, R. Siegel, E. Ward, Y. Hao, J. Xu, T. Murray, and M. J. Thun, “Cancer Statistics, 2008,” CA. Cancer J. Clin., vol. 58, no. 2, pp. 71-96, 2008, doi: 10.3322/CA.2007.0010.

[4] J. R. Mann, M. G. Backlund, and R. N. DuBois, “Mechanisms of disease: inflammatory mediators and cancer prevention,” Nat. Clin. Pract. Oncol., vol. 2, no. 4, pp. 202-210, 2005, doi: 10.1038/ncponc0140.

[5] T. Senawong, S. Khaopha, S. Misuna, J. Komaikul, G. Senawong, P. Wongphakham, and S. Yunchalard, “Phenolic acid composition and anticancer activity against human cancer cell lines of the commercially available fermentation products of Houttuynia cordata,” Sci. Asia, vol. 40, no. 1, pp. 420-427, 2014.

[6] H. -J. Lim, E. Y. Jie, I. -S. Park, S. -J. Kim, W. S. Ahn, S. -I. Jeong, S. W. Kim, and C. -H. Jung, “Anti-inflammatory effects of Weigela subsessilis callus extract via suppression of MAPK and NF-κB signaling,” Plants, vol. 10, no. 8. 2021, doi: 10.3390/plants10081635.

[7] Y. -M. Won, Z. -K. Seong, J. -L. Kim, H. -S. Kim, H. -H. Song, D. -Y. Kim, J. -H. Kim, S. -R. Oh, H. -W. Cho, J. -H. Cho, and H. -K. Lee, “Triterpene glycosides with stimulatory activity on melanogenesis from the aerial parts of Weigela subsessilis,” Arch. Pharm. Res., vol. 38, no. 8, pp. 1541-1551, 2015, doi: 10.1007/s12272-014-0524-0.

[8] C. -S. Chang, “Flavonoid chemistry of Weigela (Caprifoliaceae) in Korea,” J. Plant Res., vol. 110, no. 2, pp. 275-281, 1997, doi: 10.1007/BF02509316.

[9] N. Andriamisaina, A. -C. Mitaine-Offer, B. Pruvot, J. Chluba, T. Miyamoto, C. Tanaka, and M. -A. Lacaille-Dubois, “Phytochemistry of Weigela x ‘kosteriana variegata’ (Caprifoliaceae),” Nat. Prod. Commun., vol. 13, no. 4, pp. 403-406, 2018, doi: 10.1177/1934578X1801300406.

[10] P. T. Thuong, B.-S. Min, W. Jin, M. Na, J. Lee, R. Seong, Y. -M. Lee, K. Song, Y. Seong, H. -K. Lee, K. Bae, and S. S. Kang, “Anti-complementary activity of ursane-type triterpenoids from Weigela subsessilis,” Biol. Pharm. Bull., vol. 29, no. 4, pp. 830-833, 2006, doi: 10.1248/bpb.29.830.

[11] D. H. Nguyen, A. -C. Mitaine-Offer, S. Maroso, A. -M. Papini, T. Paululat, P. -S. Bellaye, B. Collin, O. Chambin, and M. -A. Lacaille-Dubois, “Cytotoxic glycosides from the roots of Weigela x ‘Bristol Ruby,’” Fitoterapia, vol. 137, p. 104242, 2019, doi: 10.1016/J.FITOTE.2019.104242.

[12] D. H. Nguyen, A. -C. Mitaine-Offer, T. Miyamoto, C. Tanaka, P. -S. Bellaye, B. Collin, O. Chambin, and M. -A. Lacaille-Dubois, “Phytochemical analysis of two Weigela florida cultivars, ‘Pink Poppet’ and ‘Jean’s Gold,’” Phytochem. Lett., vol. 37, pp. 85–89, 2020, doi: 10.1016/j.phytol.2020.04.009.

[13] A. N. Sennikov, D. E. Soltis, D. J. Mabberley, J. W. Byng, M. F. Fay, M. J. M. Christenhusz, M. W. Chase, P. F. Stevens, P. S. Soltis, W. S. Judd, and T. A. P. Group, “An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants: APG IV,” Bot. J. Linn. Soc., vol. 181, no. 1, pp. 1-20, 2016, doi: 10.1111/boj.12385.

[14] A. Alqethami and A. Y. Aldhebiani, “Medicinal plants used in Jeddah, Saudi Arabia: Phytochemical screening,” Saudi J. Biol. Sci., vol. 28, no. 1, pp. 805-812, 2021, doi: 10.1016/j.sjbs.2020.11.013.

[15] L. Traore, Y. Bekro, J. Pirat, and J. A. Mamyrbeva-bekro, “Study of crude extracts from Cassia sieberiana root bark and Khaya grandifoliola trunk bark: Phytochemical screening, quantitative analysis and radical scavenging activity,” Int. J. Curr. Pharm. Res., vol. 7, no. 3 - SE-Original Article(s), pp. 22-26, 2015.

[16] J. Tabart, C. Kevers, J. Pincemail, J. -O. Defraigne, and J. Dommes, “Comparative antioxidant capacities of phenolic compounds measured by various tests,” Food Chem., vol. 113, no. 4, pp. 1226-1233, 2009, doi: 10.1016/j.foodchem.2008.08.013.

[17] A. H. Cory, T. C. Owen, J. A. Barltrop, and J. G. Cory, “Use of an aqueous soluble tetrazolium/formazan assay for cell growth assays in culture,” Cancer Commun., vol. 3, no. 7, pp. 207-212, 1991.

[18] T. Murayama, A. Kasahara, Y. Shiono, and M. Ikeda, “Structure elucidation of a triterpene glycoside isolated from Weigela hortensis,” Nat. Med., vol. 57, no. 5, pp. 181-184, 2003.

[19] S. Sannigrahi, U. K. Mazuder, D. K. Pal, S. Parida, and S. Jain, “Antioxidant potential of crude extract and different fractions of Enhydra fluctuans Lour,” Iran. J. Pharm. Res. IJPR, vol. 9, no. 1, pp. 75-82, 2010.

[20] M. Na, P. T. Thuong, I. H. Hwang, K. Bae, B. Y. Kim, H. Osada, and J. S. Ahn, “Protein tyrosine phosphatase 1B inhibitory activity of 24-norursane triterpenes isolated from Weigela subsessilis,” Phyther. Res., vol. 24, no. 11, pp. 1716-1719, Nov. 2010, doi: 10.1002/ptr.3203.

[21] K. E. Heim, A. R. Tagliaferro, and D. J. Bobilya, “Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships,” J. Nutr. Biochem., vol. 13, no. 10, pp. 572-584, 2002, doi: 10.1016/S0955-2863(02)00208-5.

[22] N. Campos-Xolalpa, Á. J. Alonso-Castro, E. Sánchez-Mendoza, M. Á. Zavala-Sánchez, and S. Pérez-Gutiérrez, “Cytotoxic activity of the chloroform extract and four diterpenes isolated from Salvia ballotiflora,” Rev. Bras. Farmacogn., vol. 27, no. 3, pp. 302-305, 2017, doi: 10.1016/j.bjp.2017.01.007.

[23] J. P. Ferrer, I. C. Zampini, A. S. Cuello, M. Francisco, A. Romero, D. Valdivia, M. Gonzalez, C. Salas, A. S. Lamar, and M. I. Isla, “Cytotoxic compounds from aerial organs of Xanthium Strumarium,” Nat. Prod. Commun., vol. 11, no. 3, p. 1934578X1601100313, 2016, doi: 10.1177/1934578X1601100313.

DOI: https://doi.org/10.34238/tnu-jst.5168

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