MORPHOLOGICAL, CULTURAL CHARACTERISTIC AND NUTRITIONAL CONTENT OF Ganoderma lucidum Lim xanh COLLECTED IN LANG SON PROVINCE | Hiền | TNU Journal of Science and Technology

MORPHOLOGICAL, CULTURAL CHARACTERISTIC AND NUTRITIONAL CONTENT OF Ganoderma lucidum Lim xanh COLLECTED IN LANG SON PROVINCE

About this article

Received: 25/11/21                Revised: 20/01/22                Published: 25/01/22

Authors

1. Do Thi Hien, TNU – University of Agriculture and Forestry
2. Hoang Van Hung, TNU – University of Agriculture and Forestry
3. Do Bich Due, TNU – University of Agriculture and Forestry
4. Nguyen Manh Tuan Email to author, TNU – University of Agriculture and Forestry

Abstract


The research was carried out to collect and conserve genetic resource of Ganoderma lucidum from nature. Fruiting bodies of Ganoderma lucidum were discovered at natural forest in Huu Lan commune, Loc Binh district, Lang Son province. Analysis ofITS 1 - 5.8S rRNA - ITS 2 gene sequence showed that strain Lim xanh is a member of the genus Ganoderma and named as Ganoderma lucidum Lim xanh. Research results revealed that using 70% ethanol for 7 minutes gives the highest efficiency in isolating pure mycelium from the fruiting, with 26.67% of the samples being successful. Strain Lim xanh was ability to grow on four different media, including PDA, MCM, YMA, PL; in which the PDA medium is the most optimum for growth of  strain Lim xanh. Fruiting bodies of Ganoderma lucidum Lim xanh cultivated on artificial condition were fan-shaped, dark brown, diameter of fruit bodies reaching of 5-8 cm, stalk length 7-10 cm and 5.43 - 10.64g of fruit weight. Analysis result of nutritional contents of Ganoderma lucidum contained 15.46% total protein, carbohydrates (41.42%), fiber (37.70%) and minerals (2.45%).

Keywords


Ganoderma lucidum (Leyss. Ex Fr.) Karst; Isolation; Cultivation; Anticancer; Medical mushroom

References


[1] P. N. Nguyen, Textbook of biochemistry, part 1, Ho Chi Minh City National University Publishing House, 2001.

[2] R. R. M. Paterson, “Ganoderma- a.therapeutic funngal biofactory,” Phytochemistry, vol. 67, no. 18, pp. 1985-2001, 2006.

[3] S. Q. Huang and Z. X. Ning, “Extraction of polysaccharides from Ganoderma lucidum and its immune enhancement activity,” International Journal of Biological Macromolecules, vol. 47, no. 3, pp. 336-341, 2010.

[4] L. Feng, L. Yuan, M. Du, Y. Chen, M. H. Zhang, J. F. Gu, J. J. He, Y. Wang, and W. Cao, “Anti-lung cancer activity through enhancement of immunomodulation and induction of cell apoptosis of total triterpenes extracted from Ganoderma luccidum (Leyss. ex Fr.) Karst,” Molecules, vol. 18, no. 8, pp. 9966–9981, 2013.

[5] Z. B. Lin, “Cellular and molecular mechanisms of immuno-modulation by Ganoderma lucidum,” J Pharmacol Sci, vol. 99, no. 2, pp. 144-153, 2005.

[6] L. Z. Cao and Z. B. Lin, “Regulation on maturation and function of dendritic cells by Ganoderma lucidum polysaccharides,” Immunol Lett, vol. 83, no. 3, pp. 163-169, 2002.

[7] H. N. Zhang, J. H. He, L. Yuan, and Z. B. Lin, “In vitro and in vivo protective effect of Ganoderma lucidum polysaccharides on alloxan-induced pancreatic islets damage,” Life Sci, vol. 73, no. 18, pp. 2307-2319, 2003.

[8] H. Cao, P. P. But, and P. C. Shaw, “Methodological studies on genomic DNA extraction and purification from plant drug materials,” J Chin Pharm Sci, vol. 7, no. 3, pp. 130-137, 1998.

[9] T. J. White, T. Bruns, S. Lee, and J. Taylor, “Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics,” in PCR protocols: a guide to methods and applications, M. A. Innis, D. H. Gelfand, J. J. Sninsky, T. J. White, Ed,. San Diego (CA): Academic Press, 1990, pp. 315-322.

[10] S. Kumar, G. Stecher, and K. Tamura, “MEGA7: Molecular evolutionary eenetics analysis version 7.0 for bigger datasets,” Mol Biol Evol, vol. 33, no. 7, pp. 1870-1874, 2016.

[11] TCVN 8801:2011, Cereals and pulses - Determination of protein -nitrogen and non protein-nitrogen contents, 2011.

[12] TCVN 4594:1988, Canned foods - Determination of total sugar, reducing sugar and starch content, 1988.

[13] TCVN 9050:2012, Foodstuffs - Determination of total, soluble, and insoluble dietary fiber by enzymatic - gravimetric method, 2012.

[14] TCVN 10691:2015, Fruit and vegetable juices - Determination of ash, 2015.

[15] T. H. G. Nguyen, T. K. A. Tran, V. N. Pham, and T. T. Vu, “Breeding and cultivation of Ganoderma luccidum (Leyss. ex Fr.) Karst,” (in Vietnamese), Youth science and technology conference of agricultural, forestry and fishery schools, 2016.

[16] K. Ota, I. Yamazaki, T. Saigoku, M. Fukui, T. Miyata, K. Kamaike, T. Shirahata, F. Mizuno, Y. Asada, M. Hirotani, C. Ino, T. Yoshikawa, Y. Kobayashi, and H. Miyaoka, “Phellilane L, sesquiterpene metabolite of Phellinus linteus: Isolation, structure elucidation, and asymmetric total synthesis,” J Org Chem., vol. 82, no. 23, pp. 12377-12385, 2017.

[17] D. Vu, M. Groenewald, M. de Vries, T. Gehrmann, B. Stielow, U. Eberhardt, A. Al-Hatmi, J. Z. Groenewald, G. Cardinali, J. Houbraken, T. Boekhout, P. W. Crous, V. Robert, and G. J. M. Verkley, “Large-scale generation and analysis of filamentous fungal DNA barcodes boosts coverage for kingdom fungi and reveals thresholds for fungal species and higher taxon delimitation,” Stud Mycol, vol. 92, pp. 135-154, 2019.




DOI: https://doi.org/10.34238/tnu-jst.5299

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