INVESTIGATION OF IN VITRO ANTIFUNGAL EFFECT OF LIPID-BASED NANOEMULSIONS ENCAPSULATING GARLIC OIL, CINNAMON OIL AND NEEM OIL ON LEVEILLULA TAURICA CAUSING POWDERY MILDEW ON BELL PEPPER PLANTS

Nguyễn Minh Hiệp; Trần Thị Ngọc Mai

doi:10.34238/tnu-jst.5446

INVESTIGATION OF IN VITRO ANTIFUNGAL EFFECT OF LIPID-BASED NANOEMULSIONS ENCAPSULATING GARLIC OIL, CINNAMON OIL AND NEEM OIL ON LEVEILLULA TAURICA CAUSING POWDERY MILDEW ON BELL PEPPER PLANTS

About this article

Received: 10/01/22 Revised: 08/04/22 Published: 13/04/22

Authors

1. Nguyen Minh Hiep , Dalat nuclease research institute
2. Tran Thi Ngoc Mai, Dalat nuclease research institute

Abstract

This study aims to investigate the in vitro antifungal effect of lipid-based nanoemulsions of garlic oil (Ne-GA), cinnamon oil (Ne-CI) and neem oil (Ne-NE) against Leveillula taurica causing powdery mildew disease on bell pepper plants. Firstly, lipid-based nanoemulsions (Ne) encapsulating essential oil were prepared by a combing method of homogenization and sonication. Next, in vitro antifungal effect of Ne-GA, Ne-CI and Ne-NE was carried out by agar disk method using bell pepper leaves at dilution concentration of 25, 50, 100, 200, 400 and 600 times. The results showed that the nano-formulations of essential oils were successfully prepared with a particle size of less than 140 nm and zeta potential of more than -30 mV. The results also indicated that Ne-GA had a higher antifungal activity than Ne-CI and Ne-NE. In addition, at a dilution of 400 times, Ne-GA still exhibited the high disease-preventing and disease-treating effectiveness of 96.67% and 90%, respectively. Therefore, this study indicates that essential oil-encapsulated lipid-based nanoemulsions can be widely applied in clean and sustainable horticulture to protect bell pepper plants from Leveillula taurica.

Keywords

Nanocarriers; Leveillula taurica; Garlic essential oil; Cinnamon essential oil; Neem essential oil

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DOI: https://doi.org/10.34238/tnu-jst.5446

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