STYDY ON THE CALLUS FORMATION MEDIUM OF Turnera ulmifolia L.

Abstract

“Yellow alder” (Turnera ulmifolia L.) is well- known for a popular medical plant in the Americas with the uses to improve reproductive health, treat premature ejaculation and impotence in men. Arbutin in it has an effect on whitening our skin naturally due to the ability to inhibit enzymes, which produce melanin without harm side- effects. The extracted solution from leaves stimulates the activity of antioxidant enzymes (glutathione peroxidase, superoxide dismutase and catalase) which prevent anti-aging and free radicals, tighten and smoothen skin. This paper presents the results of studying on the callus formation medium of T.ulmifolia from Americas in order to find a multiplication process to develop this plant in Vietnam.

The suitable formula for the callus formation in leaves of T.ulmifolia is the basal MS medium supplemented with 2.0 mg/l 2.4 – D. The one in trunk fragments is the basal MS medium supplemented with 1.0 mg/l 2.4 – D in case they were cultured for one week in dark and then two week in light. Calluses formed in trunk fragments had the higher quality than in piece of leave. The basal MS medium supplemented with 2.0 mg/l BAP is good for shoot formation from callus (6.03 shoots/sample).